Direct measurement of inositol in bovine myelin basic protein

Ross Smith, Peter E. Braun, Michael A. J. Ferguson, Martin G. Low, William R. Sherman

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    Abstract

    Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.
    Original languageEnglish
    Pages (from-to)285-288
    Number of pages4
    JournalBiochemical Journal
    Volume248
    Issue number1
    Publication statusPublished - 15 Nov 1987

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