Direct measurement of inositol in bovine myelin basic protein

Ross Smith; Peter E. Braun; Michael A. J. Ferguson; Martin G. Low; William R. Sherman

Direct measurement of inositol in bovine myelin basic protein

Ross Smith
, Peter E. Braun
, Michael A. J. Ferguson
, Martin G. Low
, William R. Sherman

Research output: Contribution to journal › Article › peer-review

Abstract

Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.

Original language	English
Pages (from-to)	285-288
Number of pages	4
Journal	Biochemical Journal
Volume	248
Issue number	1
Publication status	Published - 15 Nov 1987

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title = "Direct measurement of inositol in bovine myelin basic protein",

abstract = "Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol \% of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.",

author = "Ross Smith and Braun, \{Peter E.\} and Ferguson, \{Michael A. J.\} and Low, \{Martin G.\} and Sherman, \{William R.\}",

year = "1987",

month = nov,

day = "15",

language = "English",

volume = "248",

pages = "285--288",

journal = "Biochemical Journal",

issn = "0264-6021",

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T1 - Direct measurement of inositol in bovine myelin basic protein

AU - Smith, Ross

AU - Braun, Peter E.

AU - Ferguson, Michael A. J.

AU - Low, Martin G.

AU - Sherman, William R.

PY - 1987/11/15

Y1 - 1987/11/15

N2 - Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.

AB - Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.

M3 - Article

SN - 0264-6021

VL - 248

SP - 285

EP - 288

JO - Biochemical Journal

JF - Biochemical Journal

IS - 1

ER -

Direct measurement of inositol in bovine myelin basic protein

Abstract

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