Direct observation of DNA distortion by the RSC complex

Giuseppe Lia, Elise Praly, Helder Ferreira, Chris Stockdale, Yuk Ching Tse-Dinh, David Dunlap, Vincent Croquette, David Bensimon, Tom Owen-Hughes

    Research output: Contribution to journalArticlepeer-review

    122 Citations (Scopus)

    Abstract

    The Snf2 family represents a functionally diverse class of ATPase sharing the ability to modify DNA structure. Here, we use a magnetic trap and an atomic force microscope to monitor the activity of a member of this class: the RSC complex. This enzyme caused transient shortenings in DNA length involving translocation of typically 400 bp within 2 s, resulting in the formation of a loop whose size depended on both the force applied to the DNA and the ATP concentration. The majority of loops then decrease in size within a time similar to that with which they are formed, suggesting that the motor has the ability to reverse its direction. Loop formation was also associated with the generation of negative DNA supercoils. These observations support the idea that the ATPase motors of the Snf2 family of proteins act as DNA translocases specialized to generate transient distortions in DNA structure.
    Original languageEnglish
    Pages (from-to)417-425
    Number of pages9
    JournalMolecular Cell
    Volume21
    Issue number3
    DOIs
    Publication statusPublished - Feb 2006

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