Discriminating between barley genotypes using microsatellite markers

Joanne Russell, John Fuller, George Young, Bill Thomas, Malcolm Macaulay, Robbie Waugh, Wayne Powell, Graziana Taramino

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Eleven microsatellite loci were used to survey 24 barley genotypes representing 23 cultivars and a breeding line in official trials. Three separate combinations of four microsatellites had overall probabilities of identity of less than 1 in 1000 and could distinguish between all 24 barley genotypes. It is shown that the microsatellites could distinguish genotypes with the same pedigree and also that patterns of discrimination were different from those obtained from botanical descriptors. The stability of microsatellites across different generations was demonstrated by a retrospective analysis of the pedigree of Golden Promise. One of the parents of Maythorpe, the immediate ancestor of Golden Promise, was shown to be Irish Goldthorpe rather than Goldthorpe, thereby resolving conflicting published pedigrees.

Original languageEnglish
Pages (from-to)442-450
Number of pages9
JournalGenome
Volume40
Issue number4
DOIs
Publication statusPublished - Aug 1997

Keywords

  • Barley
  • Cultivar identification
  • Golden Promise
  • Microsatellites
  • Stability

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    Russell, J., Fuller, J., Young, G., Thomas, B., Macaulay, M., Waugh, R., Powell, W., & Taramino, G. (1997). Discriminating between barley genotypes using microsatellite markers. Genome, 40(4), 442-450. https://doi.org/10.1139/g97-059