Disinfection of Needless Connectors to Reduce Staphylococcus Aureus Bacterial Load

Camila Biazus-Dalcin, Patricia Kuerten Rocha, Claire M Rickard, Ana Christina Gales, Thaís Cristine Marques Sincero, Gillian Ray-Barruel, Amanda J. Ullman, Camila Biazus-Dalcin, Mavilde L. Gonçalves Pedreira

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Disinfection of needleless connectors was undertaken in vitro against Staphylococcus aureus comparing 70% isopropyl alcohol (IPA), 70% ethanol, 0.5% and 2% chlorhexidine in 70% IPA applied with gauze, and 70% IPA single-use cap (Site-Scrub®). All disinfectants reduced the bacterial load (p<0.001), especially the chlorhexidine solutions. Mechanical friction should follow guidelines.
Original languageEnglish
Pages (from-to)14-18
Number of pages5
JournalJournal of the Association for Vascular Access
Issue number2
Early online date4 Apr 2022
Publication statusPublished - Jun 2022


  • Staphylococcus aureus
  • catheter-related bloodstream infection
  • needleless connector
  • vascular access device
  • disinfection


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