Disruption of the murine calpain small subunit gene, Capn4: calpain is essential for embryonic development but not for cell growth and division

J. Simon C. Arthur, John S. Elce, Carol Hegadorn, Karen Williams, Peter A. Greer

    Research output: Contribution to journalArticle

    281 Citations (Scopus)

    Abstract

    Calpains are a family of Ca2+-dependent intracellular cysteine proteases, including the ubiquitously expressed µ- and m-calpains. Both µ- and m-calpains are heterodimers, consisting of a distinct large 80-kDa catalytic subunit, encoded by the genes Capn1 and Capn2, and a common small 28-kDa regulatory subunit (Capn4). The physiological roles and possible functional distinctions of µ- and m-calpains remain unclear, but suggested functions include participation in cell division and migration, integrin-mediated signal transduction, apoptosis, and regulation of cellular control proteins such as cyclin D1 and p53. Homozygous disruption of murine Capn4 eliminated both µ- and m-calpain activities, but this did not affect survival and proliferation of cultured embryonic stem cells or embryonic fibroblasts, or the early stages of organogenesis. However, mutant embryos died at midgestation and displayed defects in the cardiovascular system, hemorrhaging, and accumulation of erythroid progenitors.

    Original languageEnglish
    Pages (from-to)4474-4481
    Number of pages8
    JournalMolecular and Cellular Biology
    Volume20
    Issue number12
    Publication statusPublished - Jun 2000

    Keywords

    • Animals
    • Embryonic and Fetal Development
    • Calpain
    • Mice
    • Gene Expression Regulation, Developmental
    • Gene Deletion
    • Cell Division

    Fingerprint Dive into the research topics of 'Disruption of the murine calpain small subunit gene, Capn4: calpain is essential for embryonic development but not for cell growth and division'. Together they form a unique fingerprint.

  • Cite this