Distribution of glutathione S-transferase isoenzymes in human kidney: basis for possible markers of renal injury

D J Harrison, R Kharbanda, D S Cunningham, L I McLellan, J D Hayes

    Research output: Contribution to journalArticle

    93 Citations (Scopus)

    Abstract

    To determine whether the tissue distribution of glutathione S-transferase (GST) isoenzymes could define the precise nature of renal injury, 13 adult kidneys were studied, using specific antibodies raised against purified isoenzymes. Basic GST stained strongly proximal convoluted tubules and some medullary tubules; acidic GST stained strongly distal convoluted tubules and medullary tubules; neutral GST stained similarly to acidic GST, but weaker, and microsomal GST stained glomerular and interstitial endothelium and collecting ducts deep in the medulla, although there was considerable variation in staining intensity among cases. It is suggested that the measurement of these isoenzymes in serum and urine may help to elucidate the localisation of tissue damage, which may be particularly valuable in patients with cyclosporine toxicity following renal transplantation.

    Original languageEnglish
    Pages (from-to)624-8
    Number of pages5
    JournalJournal of Clinical Pathology
    Volume42
    Issue number6
    DOIs
    Publication statusPublished - Jun 1989

    Fingerprint

    Glutathione Transferase
    Isoenzymes
    Kidney
    Wounds and Injuries
    Tissue Distribution
    Kidney Transplantation
    Cyclosporine
    Endothelium
    Urine
    Staining and Labeling
    Antibodies
    Serum

    Keywords

    • Glutathione Transferase/metabolism
    • Humans
    • Isoenzymes/metabolism
    • Kidney/anatomy & histology
    • Kidney Diseases/diagnosis
    • Microsomes/enzymology

    Cite this

    Harrison, D J ; Kharbanda, R ; Cunningham, D S ; McLellan, L I ; Hayes, J D. / Distribution of glutathione S-transferase isoenzymes in human kidney : basis for possible markers of renal injury. In: Journal of Clinical Pathology. 1989 ; Vol. 42, No. 6. pp. 624-8.
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    abstract = "To determine whether the tissue distribution of glutathione S-transferase (GST) isoenzymes could define the precise nature of renal injury, 13 adult kidneys were studied, using specific antibodies raised against purified isoenzymes. Basic GST stained strongly proximal convoluted tubules and some medullary tubules; acidic GST stained strongly distal convoluted tubules and medullary tubules; neutral GST stained similarly to acidic GST, but weaker, and microsomal GST stained glomerular and interstitial endothelium and collecting ducts deep in the medulla, although there was considerable variation in staining intensity among cases. It is suggested that the measurement of these isoenzymes in serum and urine may help to elucidate the localisation of tissue damage, which may be particularly valuable in patients with cyclosporine toxicity following renal transplantation.",
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    Distribution of glutathione S-transferase isoenzymes in human kidney : basis for possible markers of renal injury. / Harrison, D J; Kharbanda, R; Cunningham, D S; McLellan, L I; Hayes, J D.

    In: Journal of Clinical Pathology, Vol. 42, No. 6, 06.1989, p. 624-8.

    Research output: Contribution to journalArticle

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    T1 - Distribution of glutathione S-transferase isoenzymes in human kidney

    T2 - basis for possible markers of renal injury

    AU - Harrison, D J

    AU - Kharbanda, R

    AU - Cunningham, D S

    AU - McLellan, L I

    AU - Hayes, J D

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    AB - To determine whether the tissue distribution of glutathione S-transferase (GST) isoenzymes could define the precise nature of renal injury, 13 adult kidneys were studied, using specific antibodies raised against purified isoenzymes. Basic GST stained strongly proximal convoluted tubules and some medullary tubules; acidic GST stained strongly distal convoluted tubules and medullary tubules; neutral GST stained similarly to acidic GST, but weaker, and microsomal GST stained glomerular and interstitial endothelium and collecting ducts deep in the medulla, although there was considerable variation in staining intensity among cases. It is suggested that the measurement of these isoenzymes in serum and urine may help to elucidate the localisation of tissue damage, which may be particularly valuable in patients with cyclosporine toxicity following renal transplantation.

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    KW - Humans

    KW - Isoenzymes/metabolism

    KW - Kidney/anatomy & histology

    KW - Kidney Diseases/diagnosis

    KW - Microsomes/enzymology

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