Diversity and genetic differentiation among subpopulations of Guricidia sepium revealed by PCR-based assays

I. K. Dawson, A. J. Simons, R. Waugh, W. Powell

Research output: Contribution to journalArticlepeer-review

112 Citations (Scopus)

Abstract

Randomly amplified polymorphic DNA (RAPD), and a mitochondrial marker based on amplification of the V7 region of the mitochondrial small ribosomal RNA (srRNA) gene, were used to partition genetic variation within a single population of Gliricidia sepium sampled from Guatemala. Seventeen per cent of the variation detected with RAPDs was partitioned among subpopulations and indicated a greater level of discrimination than previously detected with isozymes. Cluster analysis indicated a direct relationship between this variation and the geographical distance between subpopulations. A polymorphism identified within the maternally inherited mitochondrial V7 srRNA product, which relied on digestion with restriction endonucleases, confirmed the genetic subdivision identified with RAPDs, and suggested a relatively limited role for seed in gene dispersal.

Original languageEnglish
Pages (from-to)10-18
Number of pages9
JournalHeredity
Volume74
Issue number1
DOIs
Publication statusPublished - 1 Jan 1995

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