Division of labour between PP2A-B56 isoforms at the centromere and kinetochore

Giulia Vallardi, Lindsey Allan, Lisa Crozier, Adrian T. Saurin (Lead / Corresponding author)

Research output: Contribution to journalArticle

2 Citations (Scopus)
84 Downloads (Pure)

Abstract

PP2A-B56 is a serine/threonine phosphatase complex that regulates several major mitotic processes, including sister chromatid cohesion, kinetochore-microtubule attachment and the spindle assembly checkpoint. We show here that these key functions are divided between different B56 isoforms that localise to either the centromere or kinetochore. The centromeric isoforms rely on a specific interaction with Sgo2, whereas the kinetochore isoforms bind preferentially to BubR1 and other proteins containing an LxxIxE motif. In addition to these selective binding partners, Sgo1 helps to anchor PP2A-B56 at both locations: it collaborates with BubR1 to maintain B56 at the kinetochore and it helps to preserve the Sgo2/B56 complex at the centromere. A series of chimaeras were generated to map the critical region in B56 down to a small C-terminal loop that regulates the key interactions and defines B56 localisation. Together, this study describes how different PP2A-B56 complexes utilise isoform-specific interactions to control distinct processes during mitosis.
Original languageEnglish
Article numbere42619
Pages (from-to)1-25
Number of pages25
JournaleLife
Volume8
DOIs
Publication statusPublished - 4 Mar 2019

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Kinetochores
Centromere
Protein Isoforms
Personnel
M Phase Cell Cycle Checkpoints
Chromatids
Phosphoprotein Phosphatases
Anchors
Mitosis
Microtubules
Proteins

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title = "Division of labour between PP2A-B56 isoforms at the centromere and kinetochore",
abstract = "PP2A-B56 is a serine/threonine phosphatase complex that regulates several major mitotic processes, including sister chromatid cohesion, kinetochore-microtubule attachment and the spindle assembly checkpoint. We show here that these key functions are divided between different B56 isoforms that localise to either the centromere or kinetochore. The centromeric isoforms rely on a specific interaction with Sgo2, whereas the kinetochore isoforms bind preferentially to BubR1 and other proteins containing an LxxIxE motif. In addition to these selective binding partners, Sgo1 helps to anchor PP2A-B56 at both locations: it collaborates with BubR1 to maintain B56 at the kinetochore and it helps to preserve the Sgo2/B56 complex at the centromere. A series of chimaeras were generated to map the critical region in B56 down to a small C-terminal loop that regulates the key interactions and defines B56 localisation. Together, this study describes how different PP2A-B56 complexes utilise isoform-specific interactions to control distinct processes during mitosis.",
author = "Giulia Vallardi and Lindsey Allan and Lisa Crozier and Saurin, {Adrian T.}",
note = "This work was funded by Cancer Research UK (C47320/A21229 to ATS) and Tenovus Scotland.",
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Division of labour between PP2A-B56 isoforms at the centromere and kinetochore. / Vallardi, Giulia; Allan, Lindsey; Crozier, Lisa; Saurin, Adrian T. (Lead / Corresponding author).

In: eLife, Vol. 8, e42619, 04.03.2019, p. 1-25.

Research output: Contribution to journalArticle

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T1 - Division of labour between PP2A-B56 isoforms at the centromere and kinetochore

AU - Vallardi, Giulia

AU - Allan, Lindsey

AU - Crozier, Lisa

AU - Saurin, Adrian T.

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PY - 2019/3/4

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