DNA hybridisation of routinely processed tissue for detecting HPV DNA in anal squamous cell carcinomas over 40 years

J. H. Scholefield, P. McIntyre, J. G. Palmer, P. J. Coates, N. A. Shepherd, J. M. Northover

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    To study possible changes in the incidence of human papillomavirus (HPV) associated anal squamous cell carcinomas (SCC) a simple, rapid, and sensitive technique (alkaline hydrolysis) to permit DNA hybridisation from formalin fixed, paraffin wax embedded tissue was developed. The sensitivity and specificity of the technique were established by comparison with Southern blot analysis and in situ hybridisation on the same tissue specimens. Ninety tissue specimens in a single analysis were examined using this technique. Alkaline hydrolysis was applied to fixed tissue samples which showed a two-fold increase over the past 10 years in the percentage of anal cancers containing HPV type 16 DNA when compared with the previous 30 years using 207 cases of anal cancer collected over a 40 year period. This method has several advantages over the polymerase chain reaction as it is simple, relatively inexpensive, and may be widely applied to the detection and quantification of DNA sequences, including cellular oncogenes.
    Original languageEnglish
    Pages (from-to)133-6
    Number of pages4
    JournalJournal of Clinical Pathology
    Volume43
    Issue number2
    Publication statusPublished - Feb 1990

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    Squamous Cell Carcinoma
    Anus Neoplasms
    DNA
    Hydrolysis
    Human papillomavirus 16
    Waxes
    Southern Blotting
    Oncogenes
    Paraffin
    Formaldehyde
    In Situ Hybridization
    Sensitivity and Specificity
    Polymerase Chain Reaction
    Incidence

    Cite this

    Scholefield, J. H., McIntyre, P., Palmer, J. G., Coates, P. J., Shepherd, N. A., & Northover, J. M. (1990). DNA hybridisation of routinely processed tissue for detecting HPV DNA in anal squamous cell carcinomas over 40 years. Journal of Clinical Pathology, 43(2), 133-6.
    Scholefield, J. H. ; McIntyre, P. ; Palmer, J. G. ; Coates, P. J. ; Shepherd, N. A. ; Northover, J. M. / DNA hybridisation of routinely processed tissue for detecting HPV DNA in anal squamous cell carcinomas over 40 years. In: Journal of Clinical Pathology. 1990 ; Vol. 43, No. 2. pp. 133-6.
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    title = "DNA hybridisation of routinely processed tissue for detecting HPV DNA in anal squamous cell carcinomas over 40 years",
    abstract = "To study possible changes in the incidence of human papillomavirus (HPV) associated anal squamous cell carcinomas (SCC) a simple, rapid, and sensitive technique (alkaline hydrolysis) to permit DNA hybridisation from formalin fixed, paraffin wax embedded tissue was developed. The sensitivity and specificity of the technique were established by comparison with Southern blot analysis and in situ hybridisation on the same tissue specimens. Ninety tissue specimens in a single analysis were examined using this technique. Alkaline hydrolysis was applied to fixed tissue samples which showed a two-fold increase over the past 10 years in the percentage of anal cancers containing HPV type 16 DNA when compared with the previous 30 years using 207 cases of anal cancer collected over a 40 year period. This method has several advantages over the polymerase chain reaction as it is simple, relatively inexpensive, and may be widely applied to the detection and quantification of DNA sequences, including cellular oncogenes.",
    author = "Scholefield, {J. H.} and P. McIntyre and Palmer, {J. G.} and Coates, {P. J.} and Shepherd, {N. A.} and Northover, {J. M.}",
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    Scholefield, JH, McIntyre, P, Palmer, JG, Coates, PJ, Shepherd, NA & Northover, JM 1990, 'DNA hybridisation of routinely processed tissue for detecting HPV DNA in anal squamous cell carcinomas over 40 years', Journal of Clinical Pathology, vol. 43, no. 2, pp. 133-6.

    DNA hybridisation of routinely processed tissue for detecting HPV DNA in anal squamous cell carcinomas over 40 years. / Scholefield, J. H.; McIntyre, P.; Palmer, J. G.; Coates, P. J.; Shepherd, N. A.; Northover, J. M.

    In: Journal of Clinical Pathology, Vol. 43, No. 2, 02.1990, p. 133-6.

    Research output: Contribution to journalArticle

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    T1 - DNA hybridisation of routinely processed tissue for detecting HPV DNA in anal squamous cell carcinomas over 40 years

    AU - Scholefield, J. H.

    AU - McIntyre, P.

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    AU - Coates, P. J.

    AU - Shepherd, N. A.

    AU - Northover, J. M.

    PY - 1990/2

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    N2 - To study possible changes in the incidence of human papillomavirus (HPV) associated anal squamous cell carcinomas (SCC) a simple, rapid, and sensitive technique (alkaline hydrolysis) to permit DNA hybridisation from formalin fixed, paraffin wax embedded tissue was developed. The sensitivity and specificity of the technique were established by comparison with Southern blot analysis and in situ hybridisation on the same tissue specimens. Ninety tissue specimens in a single analysis were examined using this technique. Alkaline hydrolysis was applied to fixed tissue samples which showed a two-fold increase over the past 10 years in the percentage of anal cancers containing HPV type 16 DNA when compared with the previous 30 years using 207 cases of anal cancer collected over a 40 year period. This method has several advantages over the polymerase chain reaction as it is simple, relatively inexpensive, and may be widely applied to the detection and quantification of DNA sequences, including cellular oncogenes.

    AB - To study possible changes in the incidence of human papillomavirus (HPV) associated anal squamous cell carcinomas (SCC) a simple, rapid, and sensitive technique (alkaline hydrolysis) to permit DNA hybridisation from formalin fixed, paraffin wax embedded tissue was developed. The sensitivity and specificity of the technique were established by comparison with Southern blot analysis and in situ hybridisation on the same tissue specimens. Ninety tissue specimens in a single analysis were examined using this technique. Alkaline hydrolysis was applied to fixed tissue samples which showed a two-fold increase over the past 10 years in the percentage of anal cancers containing HPV type 16 DNA when compared with the previous 30 years using 207 cases of anal cancer collected over a 40 year period. This method has several advantages over the polymerase chain reaction as it is simple, relatively inexpensive, and may be widely applied to the detection and quantification of DNA sequences, including cellular oncogenes.

    M3 - Article

    VL - 43

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    EP - 136

    JO - Journal of Clinical Pathology

    JF - Journal of Clinical Pathology

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    Scholefield JH, McIntyre P, Palmer JG, Coates PJ, Shepherd NA, Northover JM. DNA hybridisation of routinely processed tissue for detecting HPV DNA in anal squamous cell carcinomas over 40 years. Journal of Clinical Pathology. 1990 Feb;43(2):133-6.