DNA recovery from biological material on mini tapes using a simple extraction buffer and solid phase reversible immobilisation (SPRI) purification

Aga Kuffel (Lead / Corresponding author), Niamh Nic Daeid, Alexander Gray

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Abstract

In this study, we compare the performance of a simple PVP extraction method with a commercially available and widely used kit for recovering DNA from adhesive tapes. This novel method shows almost 60% higher DNA recovery from blood deposits on SceneSafe Fast™ minitapes when compared to the PrepFiler™ BTA Forensic DNA Extraction Kit. We also demonstrate how a simple modification of the magnetic bead-based purification step can lead to better recovery and removal of PCR inhibitors.

Original languageEnglish
Article number100350
Number of pages6
JournalForensic Science International
Volume9
Early online date23 Dec 2023
DOIs
Publication statusE-pub ahead of print - 23 Dec 2023

Keywords

  • DNA
  • Tape lift
  • SPRI beads
  • Purification
  • PCR inhibitors

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