DNA recovery from biological material on mini tapes using a simple extraction buffer and solid phase reversible immobilisation (SPRI) purification

Aga Kuffel; Niamh Nic Daeid; Alexander Gray

doi:10.1016/j.fsir.2023.100350

DNA recovery from biological material on mini tapes using a simple extraction buffer and solid phase reversible immobilisation (SPRI) purification

Aga Kuffel (Lead / Corresponding author), Niamh Nic Daeid, Alexander Gray

Leverhulme Research Centre for Forensic Science

Research output: Contribution to journal › Article › peer-review

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Abstract

In this study, we compare the performance of a simple PVP extraction method with a commercially available and widely used kit for recovering DNA from adhesive tapes. This novel method shows almost 60% higher DNA recovery from blood deposits on SceneSafe Fast™ minitapes when compared to the PrepFiler™ BTA Forensic DNA Extraction Kit. We also demonstrate how a simple modification of the magnetic bead-based purification step can lead to better recovery and removal of PCR inhibitors.

Original language	English
Article number	100350
Number of pages	6
Journal	Forensic Science International
Volume	9
Early online date	23 Dec 2023
DOIs	https://doi.org/10.1016/j.fsir.2023.100350
Publication status	Published - Jul 2024

Keywords

DNA
Tape lift
SPRI beads
Purification
PCR inhibitors

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10.1016/j.fsir.2023.100350Licence: CC BY-NC-ND

Final Published Version Final published version, 727 KBLicence: CC BY-NC-ND

Leverhulme Research Centre for Forensic Science (LRCFS)
Nic Daeid, N. (Investigator)
Leverhulme Trust
1/07/16 → 30/06/26
Project: Research

Orkney Science Festival - Disentangling DNA Public Talk
Gray, A. (Presenter)
10 Sept 2024
Activity: Other activity types › Public engagement and outreach - festival/exhibition

Cite this

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title = "DNA recovery from biological material on mini tapes using a simple extraction buffer and solid phase reversible immobilisation (SPRI) purification",

abstract = "In this study, we compare the performance of a simple PVP extraction method with a commercially available and widely used kit for recovering DNA from adhesive tapes. This novel method shows almost 60% higher DNA recovery from blood deposits on SceneSafe Fast{\texttrademark} minitapes when compared to the PrepFiler{\texttrademark} BTA Forensic DNA Extraction Kit. We also demonstrate how a simple modification of the magnetic bead-based purification step can lead to better recovery and removal of PCR inhibitors.",

keywords = "DNA, Tape lift, SPRI beads, Purification, PCR inhibitors",

author = "Aga Kuffel and {Nic Daeid}, Niamh and Alexander Gray",

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doi = "10.1016/j.fsir.2023.100350",

language = "English",

volume = "9",

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AU - Kuffel, Aga

AU - Nic Daeid, Niamh

AU - Gray, Alexander

PY - 2024/7

Y1 - 2024/7

N2 - In this study, we compare the performance of a simple PVP extraction method with a commercially available and widely used kit for recovering DNA from adhesive tapes. This novel method shows almost 60% higher DNA recovery from blood deposits on SceneSafe Fast™ minitapes when compared to the PrepFiler™ BTA Forensic DNA Extraction Kit. We also demonstrate how a simple modification of the magnetic bead-based purification step can lead to better recovery and removal of PCR inhibitors.

AB - In this study, we compare the performance of a simple PVP extraction method with a commercially available and widely used kit for recovering DNA from adhesive tapes. This novel method shows almost 60% higher DNA recovery from blood deposits on SceneSafe Fast™ minitapes when compared to the PrepFiler™ BTA Forensic DNA Extraction Kit. We also demonstrate how a simple modification of the magnetic bead-based purification step can lead to better recovery and removal of PCR inhibitors.

KW - DNA

KW - Tape lift

KW - SPRI beads

KW - Purification

KW - PCR inhibitors

U2 - 10.1016/j.fsir.2023.100350

DO - 10.1016/j.fsir.2023.100350

M3 - Article

SN - 0379-0738

VL - 9

JO - Forensic Science International

JF - Forensic Science International

M1 - 100350

ER -

DNA recovery from biological material on mini tapes using a simple extraction buffer and solid phase reversible immobilisation (SPRI) purification

Abstract

Keywords

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Projects

Leverhulme Research Centre for Forensic Science (LRCFS)

Activities

Orkney Science Festival - Disentangling DNA Public Talk

Cite this