DNA transposition by the RAG1 and RAG2 proteins: a possible source of oncogenic translocations

Kevin Hiom; Meni Melek; Martin Gellert

doi:10.1016/S0092-8674(00)81587-1

DNA transposition by the RAG1 and RAG2 proteins: a possible source of oncogenic translocations

Kevin Hiom, Meni Melek, Martin Gellert (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

441 Citations (Scopus)

Abstract

The RAG1 and RAG2 proteins are known to initiate V(D)J recombination by making a double-strand break between the recombination signal sequence (RSS) and the neighboring coding DNA. We show that these proteins can also drive the coupled insertion of cleaved recombination signals into new DNA sites in a transpositional reaction. This RAG-mediated DNA transfer provides strong evidence for the evolution of the V(D)J recombination system from an ancient mobile DNA element and suggests that repeated transposition may have promoted the expansion of the antigen receptor loci. The inappropriate diversion of V(D)J rearrangement to a transpositional pathway may also help to explain certain types of DNA translocation associated with lymphatic tumors.

Original language	English
Pages (from-to)	463-470
Number of pages	8
Journal	Cell
Volume	94
Issue number	4
DOIs	https://doi.org/10.1016/S0092-8674(00)81587-1
Publication status	Published - 21 Aug 1998

Keywords

DNA
DNA Nucleotidyltransferases
DNA Transposable Elements
DNA-Binding Proteins
Evolution, Molecular
Gene Rearrangement, T-Lymphocyte
Homeodomain Proteins
Lymphoma
Models, Genetic
Nucleotidyltransferases
Oncogenes
Receptors, Antigen, T-Cell
Recombination, Genetic
Translocation, Genetic
Transposases
VDJ Recombinases

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10.1016/S0092-8674(00)81587-1

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title = "DNA transposition by the RAG1 and RAG2 proteins: a possible source of oncogenic translocations",

abstract = "The RAG1 and RAG2 proteins are known to initiate V(D)J recombination by making a double-strand break between the recombination signal sequence (RSS) and the neighboring coding DNA. We show that these proteins can also drive the coupled insertion of cleaved recombination signals into new DNA sites in a transpositional reaction. This RAG-mediated DNA transfer provides strong evidence for the evolution of the V(D)J recombination system from an ancient mobile DNA element and suggests that repeated transposition may have promoted the expansion of the antigen receptor loci. The inappropriate diversion of V(D)J rearrangement to a transpositional pathway may also help to explain certain types of DNA translocation associated with lymphatic tumors.",

keywords = "DNA, DNA Nucleotidyltransferases, DNA Transposable Elements, DNA-Binding Proteins, Evolution, Molecular, Gene Rearrangement, T-Lymphocyte, Homeodomain Proteins, Lymphoma, Models, Genetic, Nucleotidyltransferases, Oncogenes, Receptors, Antigen, T-Cell, Recombination, Genetic, Translocation, Genetic, Transposases, VDJ Recombinases",

author = "Kevin Hiom and Meni Melek and Martin Gellert",

year = "1998",

month = aug,

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doi = "10.1016/S0092-8674(00)81587-1",

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pages = "463--470",

journal = "Cell",

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TY - JOUR

T1 - DNA transposition by the RAG1 and RAG2 proteins

T2 - a possible source of oncogenic translocations

AU - Hiom, Kevin

AU - Melek, Meni

AU - Gellert, Martin

PY - 1998/8/21

Y1 - 1998/8/21

N2 - The RAG1 and RAG2 proteins are known to initiate V(D)J recombination by making a double-strand break between the recombination signal sequence (RSS) and the neighboring coding DNA. We show that these proteins can also drive the coupled insertion of cleaved recombination signals into new DNA sites in a transpositional reaction. This RAG-mediated DNA transfer provides strong evidence for the evolution of the V(D)J recombination system from an ancient mobile DNA element and suggests that repeated transposition may have promoted the expansion of the antigen receptor loci. The inappropriate diversion of V(D)J rearrangement to a transpositional pathway may also help to explain certain types of DNA translocation associated with lymphatic tumors.

AB - The RAG1 and RAG2 proteins are known to initiate V(D)J recombination by making a double-strand break between the recombination signal sequence (RSS) and the neighboring coding DNA. We show that these proteins can also drive the coupled insertion of cleaved recombination signals into new DNA sites in a transpositional reaction. This RAG-mediated DNA transfer provides strong evidence for the evolution of the V(D)J recombination system from an ancient mobile DNA element and suggests that repeated transposition may have promoted the expansion of the antigen receptor loci. The inappropriate diversion of V(D)J rearrangement to a transpositional pathway may also help to explain certain types of DNA translocation associated with lymphatic tumors.

KW - DNA

KW - DNA Nucleotidyltransferases

KW - DNA Transposable Elements

KW - DNA-Binding Proteins

KW - Evolution, Molecular

KW - Gene Rearrangement, T-Lymphocyte

KW - Homeodomain Proteins

KW - Lymphoma

KW - Models, Genetic

KW - Nucleotidyltransferases

KW - Oncogenes

KW - Receptors, Antigen, T-Cell

KW - Recombination, Genetic

KW - Translocation, Genetic

KW - Transposases

KW - VDJ Recombinases

U2 - 10.1016/S0092-8674(00)81587-1

DO - 10.1016/S0092-8674(00)81587-1

M3 - Article

C2 - 9727489

SN - 0092-8674

VL - 94

SP - 463

EP - 470

JO - Cell

JF - Cell

IS - 4

ER -

DNA transposition by the RAG1 and RAG2 proteins: a possible source of oncogenic translocations

Abstract

Keywords

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