Does combining magnetic-activated cell sorting with density gradient or swim-up improve sperm selection?

TY - JOUR

T1 - Does combining magnetic-activated cell sorting with density gradient or swim-up improve sperm selection?

AU - Cakar, Zeynep

AU - Cetinkaya, Burcu

AU - Aras, Duru

AU - Koca, Betül

AU - Ozkavukcu, Sinan

AU - Kaplanoglu, İskender

AU - Can, Alp

AU - Cinar, Ozgur

N1 - Funding Information: This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, 213S019) and Ankara University Scientific Research Project Coordination (AU-BAP 13O3330001). Preliminary outcomes of this study were presented in the ESHRE 2015 in Lisbon, Portugal. Publisher Copyright: © 2016, Springer Science+Business Media New York.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Purpose The present study aimed to evaluate whether combining the magnetic-activated cell sorting (MACS) with density-gradient (DG) or swim-up (SU) sperm separation techniques can improve sperm selection to obtain higher quality spermatozoa. Methods Two commonly used sperm selection techniques, SU and DG, were compared to MACS combined with either SU or DG. Spermatozoa obtained from normozoospermic (n = 10) and oligozoospermic (n = 10) cases were grouped as SU, DG, SU+MACS, and DG+MACS followed by the analysis of sperm morphology, motility, DNA integrity, and the levels of Izumo-1 and PLCZ proteins. Results Although spermatozoa obtained by SU or DG when combined with MACS have improved aspects when compared to SU or DG alone, results did not reach a statistically significant level. Moreover, separation with MACS caused a significant loss in the numbers of total and rapid progressive spermatozoa. Conclusions: Considering the cost/benefit ratio, MACS application together with traditional techniques may only be preferred in certain cases having higher concentrations of spermatozoa, but it does not seem to be an ideal and practical sperm selection technique for routine use.

AB - Purpose The present study aimed to evaluate whether combining the magnetic-activated cell sorting (MACS) with density-gradient (DG) or swim-up (SU) sperm separation techniques can improve sperm selection to obtain higher quality spermatozoa. Methods Two commonly used sperm selection techniques, SU and DG, were compared to MACS combined with either SU or DG. Spermatozoa obtained from normozoospermic (n = 10) and oligozoospermic (n = 10) cases were grouped as SU, DG, SU+MACS, and DG+MACS followed by the analysis of sperm morphology, motility, DNA integrity, and the levels of Izumo-1 and PLCZ proteins. Results Although spermatozoa obtained by SU or DG when combined with MACS have improved aspects when compared to SU or DG alone, results did not reach a statistically significant level. Moreover, separation with MACS caused a significant loss in the numbers of total and rapid progressive spermatozoa. Conclusions: Considering the cost/benefit ratio, MACS application together with traditional techniques may only be preferred in certain cases having higher concentrations of spermatozoa, but it does not seem to be an ideal and practical sperm selection technique for routine use.

KW - DNA integrity

KW - Izumo-1

KW - Magnetic cell sorting

KW - PLC-ζ

KW - Sperm selection

UR - https://www.scopus.com/pages/publications/84982806186

U2 - 10.1007/s10815-016-0742-0

DO - 10.1007/s10815-016-0742-0

M3 - Article

C2 - 27233651

AN - SCOPUS:84982806186

SN - 1058-0468

VL - 33

SP - 1059

EP - 1065

JO - Journal of Assisted Reproduction and Genetics

JF - Journal of Assisted Reproduction and Genetics

IS - 8

ER -