TY - JOUR
T1 - Downregulation of cornulin in esophageal squamous cell carcinoma
AU - Pawar, Harsh
AU - Maharudraiah, Jagadeesha
AU - Kashyap, Manoj Kumar
AU - Sharma, Jyoti
AU - Srikanth, Srinivas Manda
AU - Choudhary, Robin
AU - Chavan, Sandip
AU - Sathe, Gajanan
AU - Manju, Hosuru Chikkalingaiah
AU - Kumar, Kariyanakatte Veeraiah Veerendra
AU - Vijayakumar, Manavalan
AU - Sirdeshmukh, Ravi
AU - Harsha, Hindahally Chandregowda
AU - Prasad, Thottethodi Subrahmanya Keshava
AU - Pandey, Akhilesh
AU - Kumar, Rekha Vijay
N1 - Funding Information:
We thank the Department of Biotechnology (DBT), Government of India, for research support to the Institute of Bioinformatics. T.S.K. Prasad and Rekha V. Kumar are supported by DBT grant ( DBT/CSH/GIA/1583/2010-2011 ). T.S.K. Prasad is also a recipient of the young investigator award from DBT, India. Harsha Gowda is a Wellcome Trust/DBT India Alliance Early Career Fellow. Harsh Pawar, Jyoti Sharma are recipients of Senior Research Fellowship and Gajanan Sathe, Sandip Chavan are recipients of Junior Research Fellowship from Council of Scientific and Industrial Research (CSIR), Government of India. Srinivas M. Srikanth is a recipient of Junior Research Fellowship from University Grants Commission (UGC), Government of India. We would like to thank Drs. S.K. Shankar and Anita Mahadevan of National Institute of Mental Health and Neurological Sciences (NIMHANS), for providing access to the imaging facility.
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/3
Y1 - 2013/3
N2 - Early events in the development of esophageal squamous cell carcinoma (ESCC) are poorly understood and many of the key molecules involved have not yet been identified. We previously used isobaric tags for a relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach to identify differentially expressed proteins in ESCC tissue as compared to the adjacent normal mucosa. Cornulin was identified as one of the major downregulated molecules in ESCC. Cornulin is a member of the S100 fused-type protein family, which has an EF-hand calcium binding motif and multiple tandem repeats of specific peptide motifs. Cornulin was 5-fold downregulated in ESCC as compared to normal epithelium mirroring our previous findings in a gene expression study of ESCC. In the present study, we performed immunohistochemical validation of cornulin (CRNN) in a larger set of patients with ESCC. Downregulation of cornulin was observed in 89% (n= 239) of 266 different ESCC tissues arrayed on tissue microarrays (TMAs). Expression of cornulin was observed in the prickle and functional cell layers of normal esophageal mucosa, localized predominantly in the cytoplasm and perinuclear region. The large majority of ESCC cases had little or no expression of cornulin in the carcinoma or stroma. These findings suggest that cornulin is an important molecule in normal esophageal pathology and is likely lost during the conversion of normal to neoplastic epithelium.
AB - Early events in the development of esophageal squamous cell carcinoma (ESCC) are poorly understood and many of the key molecules involved have not yet been identified. We previously used isobaric tags for a relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach to identify differentially expressed proteins in ESCC tissue as compared to the adjacent normal mucosa. Cornulin was identified as one of the major downregulated molecules in ESCC. Cornulin is a member of the S100 fused-type protein family, which has an EF-hand calcium binding motif and multiple tandem repeats of specific peptide motifs. Cornulin was 5-fold downregulated in ESCC as compared to normal epithelium mirroring our previous findings in a gene expression study of ESCC. In the present study, we performed immunohistochemical validation of cornulin (CRNN) in a larger set of patients with ESCC. Downregulation of cornulin was observed in 89% (n= 239) of 266 different ESCC tissues arrayed on tissue microarrays (TMAs). Expression of cornulin was observed in the prickle and functional cell layers of normal esophageal mucosa, localized predominantly in the cytoplasm and perinuclear region. The large majority of ESCC cases had little or no expression of cornulin in the carcinoma or stroma. These findings suggest that cornulin is an important molecule in normal esophageal pathology and is likely lost during the conversion of normal to neoplastic epithelium.
KW - C1Orf10
KW - Epidermal differentiation complex
KW - Loss of heterozygosity
KW - Mass spectrometry
KW - S100 gene family
KW - Squamous epithelial-induced stress protein of 53kDa
KW - Stress response protein
UR - http://www.scopus.com/inward/record.url?scp=84872380651&partnerID=8YFLogxK
U2 - 10.1016/j.acthis.2012.04.003
DO - 10.1016/j.acthis.2012.04.003
M3 - Article
C2 - 22560086
AN - SCOPUS:84872380651
SN - 0065-1281
VL - 115
SP - 89
EP - 99
JO - Acta Histochemica
JF - Acta Histochemica
IS - 2
ER -