Draft genome of the filarial nematode parasite Brugia malayi

Elodie Ghedin (Lead / Corresponding author), Shiliang Wang, David Spiro, Elisabet Caler, Qi Zhao, Jonathan Crabtree, Jonathan E. Allen, Arthur L. Delcher, David B. Guiliano, Diego Miranda-Saavedra, Samuel V. Angiuoli, Todd Creasy, Paolo Amedeo, Brian Haas, Najib M. El-Sayed, Jennifer R. Wortman, Tamara Feldblyum, Luke Tallon, Michael Schatz, Martin ShumwayHean Koo, Steven L Salzberg, Seth Schobel, Mihaela Pertea, Mihai Pop, Owen White, Geoffrey J Barton, Clotilde K S Carlow, Michael J Crawford, Jennifer Daub, Matthew W Dimmic, Chris F Estes, Jeremy M Foster, Mehul Ganatra, William F Gregory, Nicholas M Johnson, Jinming Jin, Richard Komuniecki, Ian Korf, Sanjay Kumar, Sandra Laney, Ben-Wen Li, Wen Li, Tim H Lindblom, Sara Lustigman, Dong Ma, Claude V Maina, David M. A. Martin, James P. McCarter, Larry McReynolds, Makedonka Mitreva, Thomas B. Nutman, John Parkinson, José M. Peregrín-Alvarez, Catherine Poole, Qinghu Ren, Lori Saunders, Ann E. Sluder, Katherine Smith, Mario Stanke, Thomas R. Unnasch, Jenna Ware, Aguan D. Wei, Gary Weil, Deryck J. Williams, Yinhua Zhang, Steven A. Williams, Claire Fraser-Liggett, Barton Slatko, Mark L Blaxter, Alan L. Scott

    Research output: Contribution to journalArticlepeer-review

    482 Citations (Scopus)

    Abstract

    Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the approximately 90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict approximately 11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during approximately 350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design.

    Original languageEnglish
    Pages (from-to)1756-1760
    Number of pages5
    JournalScience
    Volume317
    Issue number5845
    DOIs
    Publication statusPublished - 21 Sep 2007

    Keywords

    • Animals
    • Brugia malayi
    • Caenorhabditis
    • Drosophila melanogaster
    • Drug Resistance
    • Filariasis
    • Genome, Helminth
    • Humans
    • Molecular sequence data

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