Draft genome of the filarial nematode parasite Brugia malayi

Elodie Ghedin; Shiliang Wang; David Spiro; Elisabet Caler; Qi Zhao; Jonathan Crabtree; Jonathan E. Allen; Arthur L. Delcher; David B. Guiliano; Diego Miranda-Saavedra; Samuel V. Angiuoli; Todd Creasy; Paolo Amedeo; Brian Haas; Najib M. El-Sayed; Jennifer R. Wortman; Tamara Feldblyum; Luke Tallon; Michael Schatz; Martin Shumway; Hean Koo; Steven L Salzberg; Seth Schobel; Mihaela Pertea; Mihai Pop; Owen White; Geoffrey J Barton; Clotilde K S Carlow; Michael J Crawford; Jennifer Daub; Matthew W Dimmic; Chris F Estes; Jeremy M Foster; Mehul Ganatra; William F Gregory; Nicholas M Johnson; Jinming Jin; Richard Komuniecki; Ian Korf; Sanjay Kumar; Sandra Laney; Ben-Wen Li; Wen Li; Tim H Lindblom; Sara Lustigman; Dong Ma; Claude V Maina; David M. A. Martin; James P. McCarter; Larry McReynolds; Makedonka Mitreva; Thomas B. Nutman; John  Parkinson; José M. Peregrín-Alvarez; Catherine Poole; Qinghu Ren; Lori Saunders; Ann E. Sluder; Katherine  Smith; Mario Stanke; Thomas R. Unnasch; Jenna Ware; Aguan D. Wei; Gary Weil; Deryck J. Williams; Yinhua Zhang; Steven A. Williams; Claire Fraser-Liggett; Barton Slatko; Mark L Blaxter; Alan L. Scott

doi:10.1126/science.1145406

Draft genome of the filarial nematode parasite Brugia malayi

Elodie Ghedin (Lead / Corresponding author), Shiliang Wang, David Spiro, Elisabet Caler, Qi Zhao, Jonathan Crabtree, Jonathan E. Allen, Arthur L. Delcher, David B. Guiliano, Diego Miranda-Saavedra, Samuel V. Angiuoli, Todd Creasy, Paolo Amedeo, Brian Haas, Najib M. El-Sayed, Jennifer R. Wortman, Tamara Feldblyum, Luke Tallon, Michael Schatz, Martin ShumwayHean Koo, Steven L Salzberg, Seth Schobel, Mihaela Pertea, Mihai Pop, Owen White, Geoffrey J Barton, Clotilde K S Carlow, Michael J Crawford, Jennifer Daub, Matthew W Dimmic, Chris F Estes, Jeremy M Foster, Mehul Ganatra, William F Gregory, Nicholas M Johnson, Jinming Jin, Richard Komuniecki, Ian Korf, Sanjay Kumar, Sandra Laney, Ben-Wen Li, Wen Li, Tim H Lindblom, Sara Lustigman, Dong Ma, Claude V Maina, David M. A. Martin, James P. McCarter, Larry McReynolds, Makedonka Mitreva, Thomas B. Nutman, John Parkinson, José M. Peregrín-Alvarez, Catherine Poole, Qinghu Ren, Lori Saunders, Ann E. Sluder, Katherine Smith, Mario Stanke, Thomas R. Unnasch, Jenna Ware, Aguan D. Wei, Gary Weil, Deryck J. Williams, Yinhua Zhang, Steven A. Williams, Claire Fraser-Liggett, Barton Slatko, Mark L Blaxter, Alan L. Scott

Research output: Contribution to journal › Article › peer-review

538 Citations (Scopus)

Abstract

Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the approximately 90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict approximately 11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during approximately 350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design.

Original language	English
Pages (from-to)	1756-1760
Number of pages	5
Journal	Science
Volume	317
Issue number	5845
DOIs	https://doi.org/10.1126/science.1145406
Publication status	Published - 21 Sept 2007

Keywords

Animals
Brugia malayi
Caenorhabditis
Drosophila melanogaster
Drug Resistance
Filariasis
Genome, Helminth
Humans
Molecular sequence data

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10.1126/science.1145406

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Ghedin, E., Wang, S., Spiro, D., Caler, E., Zhao, Q., Crabtree, J., Allen, J. E., Delcher, A. L., Guiliano, D. B., Miranda-Saavedra, D., Angiuoli, S. V., Creasy, T., Amedeo, P., Haas, B., El-Sayed, N. M., Wortman, J. R., Feldblyum, T., Tallon, L., Schatz, M., ... Scott, A. L. (2007). Draft genome of the filarial nematode parasite Brugia malayi. Science, 317(5845), 1756-1760. https://doi.org/10.1126/science.1145406

@article{c4d6aa28c4ed4c828db1998ad0abaa1f,

title = "Draft genome of the filarial nematode parasite Brugia malayi",

abstract = "Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the approximately 90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict approximately 11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during approximately 350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design.",

keywords = "Animals, Brugia malayi, Caenorhabditis, Drosophila melanogaster, Drug Resistance, Filariasis, Genome, Helminth, Humans, Molecular sequence data",

author = "Elodie Ghedin and Shiliang Wang and David Spiro and Elisabet Caler and Qi Zhao and Jonathan Crabtree and Allen, {Jonathan E.} and Delcher, {Arthur L.} and Guiliano, {David B.} and Diego Miranda-Saavedra and Angiuoli, {Samuel V.} and Todd Creasy and Paolo Amedeo and Brian Haas and El-Sayed, {Najib M.} and Wortman, {Jennifer R.} and Tamara Feldblyum and Luke Tallon and Michael Schatz and Martin Shumway and Hean Koo and Salzberg, {Steven L} and Seth Schobel and Mihaela Pertea and Mihai Pop and Owen White and Barton, {Geoffrey J} and Carlow, {Clotilde K S} and Crawford, {Michael J} and Jennifer Daub and Dimmic, {Matthew W} and Estes, {Chris F} and Foster, {Jeremy M} and Mehul Ganatra and Gregory, {William F} and Johnson, {Nicholas M} and Jinming Jin and Richard Komuniecki and Ian Korf and Sanjay Kumar and Sandra Laney and Ben-Wen Li and Wen Li and Lindblom, {Tim H} and Sara Lustigman and Dong Ma and Maina, {Claude V} and Martin, {David M. A.} and McCarter, {James P.} and Larry McReynolds and Makedonka Mitreva and Nutman, {Thomas B.} and John Parkinson and Peregr{\'i}n-Alvarez, {Jos{\'e} M.} and Catherine Poole and Qinghu Ren and Lori Saunders and Sluder, {Ann E.} and Katherine Smith and Mario Stanke and Unnasch, {Thomas R.} and Jenna Ware and Wei, {Aguan D.} and Gary Weil and Williams, {Deryck J.} and Yinhua Zhang and Williams, {Steven A.} and Claire Fraser-Liggett and Barton Slatko and Blaxter, {Mark L} and Scott, {Alan L.}",

year = "2007",

month = sep,

day = "21",

doi = "10.1126/science.1145406",

language = "English",

volume = "317",

pages = "1756--1760",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5845",

}

Ghedin, E, Wang, S, Spiro, D, Caler, E, Zhao, Q, Crabtree, J, Allen, JE, Delcher, AL, Guiliano, DB, Miranda-Saavedra, D, Angiuoli, SV, Creasy, T, Amedeo, P, Haas, B, El-Sayed, NM, Wortman, JR, Feldblyum, T, Tallon, L, Schatz, M, Shumway, M, Koo, H, Salzberg, SL, Schobel, S, Pertea, M, Pop, M, White, O, Barton, GJ, Carlow, CKS, Crawford, MJ, Daub, J, Dimmic, MW, Estes, CF, Foster, JM, Ganatra, M, Gregory, WF, Johnson, NM, Jin, J, Komuniecki, R, Korf, I, Kumar, S, Laney, S, Li, B-W, Li, W, Lindblom, TH, Lustigman, S, Ma, D, Maina, CV, Martin, DMA, McCarter, JP, McReynolds, L, Mitreva, M, Nutman, TB, Parkinson, J, Peregrín-Alvarez, JM, Poole, C, Ren, Q, Saunders, L, Sluder, AE, Smith, K, Stanke, M, Unnasch, TR, Ware, J, Wei, AD, Weil, G, Williams, DJ, Zhang, Y, Williams, SA, Fraser-Liggett, C, Slatko, B, Blaxter, ML & Scott, AL 2007, 'Draft genome of the filarial nematode parasite Brugia malayi', Science, vol. 317, no. 5845, pp. 1756-1760. https://doi.org/10.1126/science.1145406

TY - JOUR

T1 - Draft genome of the filarial nematode parasite Brugia malayi

AU - Ghedin, Elodie

AU - Wang, Shiliang

AU - Spiro, David

AU - Caler, Elisabet

AU - Zhao, Qi

AU - Crabtree, Jonathan

AU - Allen, Jonathan E.

AU - Delcher, Arthur L.

AU - Guiliano, David B.

AU - Miranda-Saavedra, Diego

AU - Angiuoli, Samuel V.

AU - Creasy, Todd

AU - Amedeo, Paolo

AU - Haas, Brian

AU - El-Sayed, Najib M.

AU - Wortman, Jennifer R.

AU - Feldblyum, Tamara

AU - Tallon, Luke

AU - Schatz, Michael

AU - Shumway, Martin

AU - Koo, Hean

AU - Salzberg, Steven L

AU - Schobel, Seth

AU - Pertea, Mihaela

AU - Pop, Mihai

AU - White, Owen

AU - Barton, Geoffrey J

AU - Carlow, Clotilde K S

AU - Crawford, Michael J

AU - Daub, Jennifer

AU - Dimmic, Matthew W

AU - Estes, Chris F

AU - Foster, Jeremy M

AU - Ganatra, Mehul

AU - Gregory, William F

AU - Johnson, Nicholas M

AU - Jin, Jinming

AU - Komuniecki, Richard

AU - Korf, Ian

AU - Kumar, Sanjay

AU - Laney, Sandra

AU - Li, Ben-Wen

AU - Li, Wen

AU - Lindblom, Tim H

AU - Lustigman, Sara

AU - Ma, Dong

AU - Maina, Claude V

AU - Martin, David M. A.

AU - McCarter, James P.

AU - McReynolds, Larry

AU - Mitreva, Makedonka

AU - Nutman, Thomas B.

AU - Parkinson, John

AU - Peregrín-Alvarez, José M.

AU - Poole, Catherine

AU - Ren, Qinghu

AU - Saunders, Lori

AU - Sluder, Ann E.

AU - Smith, Katherine

AU - Stanke, Mario

AU - Unnasch, Thomas R.

AU - Ware, Jenna

AU - Wei, Aguan D.

AU - Weil, Gary

AU - Williams, Deryck J.

AU - Zhang, Yinhua

AU - Williams, Steven A.

AU - Fraser-Liggett, Claire

AU - Slatko, Barton

AU - Blaxter, Mark L

AU - Scott, Alan L.

PY - 2007/9/21

Y1 - 2007/9/21

N2 - Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the approximately 90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict approximately 11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during approximately 350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design.

AB - Parasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the approximately 90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict approximately 11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during approximately 350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design.

KW - Animals

KW - Brugia malayi

KW - Caenorhabditis

KW - Drosophila melanogaster

KW - Drug Resistance

KW - Filariasis

KW - Genome, Helminth

KW - Humans

KW - Molecular sequence data

U2 - 10.1126/science.1145406

DO - 10.1126/science.1145406

M3 - Article

C2 - 17885136

SN - 0036-8075

VL - 317

SP - 1756

EP - 1760

JO - Science

JF - Science

IS - 5845

ER -

Draft genome of the filarial nematode parasite Brugia malayi

Abstract

Keywords

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