Drosophila tubulin-binding cofactor B is required for microtubule network formation and for cell polarity

Alexandre D Baffet, Béatrice Benoit, Jens Januschke, Jennifer Audo, Vanessa Gourhand, Siegfried Roth, Antoine Guichet

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Microtubules (MTs) are essential for cell division, shape, intracellular transport, and polarity. MT stability is regulated by many factors, including MT-associated proteins and proteins controlling the amount of free tubulin heterodimers available for polymerization. Tubulin-binding cofactors are potential key regulators of free tubulin concentration, since they are required for a-ß-tubulin dimerization in vitro. In this paper, we show that mutation of the Drosophila tubulin-binding cofactor B (dTBCB) affects the levels of both a- and ß-tubulins and dramatically destabilizes the MT network in different fly tissues. However, we find that dTBCB is dispensable for the early MT-dependent steps of oogenesis, including cell division, and that dTBCB is not required for mitosis in several tissues. In striking contrast, the absence of dTBCB during later stages of oogenesis causes major defects in cell polarity. We show that dTBCB is required for the polarized localization of the axis-determining mRNAs within the oocyte and for the apico-basal polarity of the surrounding follicle cells. These results establish a developmental function for the dTBCB gene that is essential for viability and MT-dependent cell polarity, but not cell division.
    Original languageEnglish
    Pages (from-to)3591-601
    Number of pages11
    JournalMolecular Biology of the Cell
    Volume23
    Issue number18
    DOIs
    Publication statusPublished - 2012

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