Dynamic properties of nucleosomes during thermal and ATP-driven mobilization

Andrew Flaus, Tom Owen-Hughes

    Research output: Contribution to journalArticle

    75 Citations (Scopus)

    Abstract

    The fundamental subunit of chromatin, the nucleosome, is not a static entity but can move along DNA via either thermal or enzyme-driven movements. Here we have monitored the movements of nucleosomes following deposition at well-defined locations on mouse mammary tumor virus promoter DNA. We found that the sites to which nucleosomes are deposited during chromatin assembly differ from those favored during thermal equilibration. Taking advantage of this, we were able to track the movement of nucleosomes over 156 bp and found that this proceeds via intermediate positions spaced between 46 and 62 bp. The remodeling enzyme ISWI was found to direct the movement of nucleosomes to sites related to those observed during thermal mobilization. In contrast, nucleosome mobilization driven by the SWI/SNF and RSC complexes were found to drive nucleosomes towards sites up to 51 bp beyond DNA ends, with little respect for the sites favored during thermal repositioning. The dynamic properties of nucleosomes we describe are likely to influence their role in gene regulation.
    Original languageEnglish
    Pages (from-to)7767-7779
    Number of pages13
    JournalMolecular and Cellular Biology
    Volume23
    Issue number21
    DOIs
    Publication statusPublished - 2003

    Fingerprint

    Nucleosomes
    Hot Temperature
    Adenosine Triphosphate
    DNA
    Mouse mammary tumor virus
    Chromatin Assembly and Disassembly
    Enzymes
    Carcinogens
    Chromatin

    Keywords

    • Chromatin
    • Nucleosomes
    • Enzyme mobilization
    • Thermal mobilization

    Cite this

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    abstract = "The fundamental subunit of chromatin, the nucleosome, is not a static entity but can move along DNA via either thermal or enzyme-driven movements. Here we have monitored the movements of nucleosomes following deposition at well-defined locations on mouse mammary tumor virus promoter DNA. We found that the sites to which nucleosomes are deposited during chromatin assembly differ from those favored during thermal equilibration. Taking advantage of this, we were able to track the movement of nucleosomes over 156 bp and found that this proceeds via intermediate positions spaced between 46 and 62 bp. The remodeling enzyme ISWI was found to direct the movement of nucleosomes to sites related to those observed during thermal mobilization. In contrast, nucleosome mobilization driven by the SWI/SNF and RSC complexes were found to drive nucleosomes towards sites up to 51 bp beyond DNA ends, with little respect for the sites favored during thermal repositioning. The dynamic properties of nucleosomes we describe are likely to influence their role in gene regulation.",
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    Dynamic properties of nucleosomes during thermal and ATP-driven mobilization. / Flaus, Andrew; Owen-Hughes, Tom.

    In: Molecular and Cellular Biology, Vol. 23, No. 21, 2003, p. 7767-7779.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Dynamic properties of nucleosomes during thermal and ATP-driven mobilization

    AU - Flaus, Andrew

    AU - Owen-Hughes, Tom

    N1 - dc.publisher: American Society for Microbiology

    PY - 2003

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    N2 - The fundamental subunit of chromatin, the nucleosome, is not a static entity but can move along DNA via either thermal or enzyme-driven movements. Here we have monitored the movements of nucleosomes following deposition at well-defined locations on mouse mammary tumor virus promoter DNA. We found that the sites to which nucleosomes are deposited during chromatin assembly differ from those favored during thermal equilibration. Taking advantage of this, we were able to track the movement of nucleosomes over 156 bp and found that this proceeds via intermediate positions spaced between 46 and 62 bp. The remodeling enzyme ISWI was found to direct the movement of nucleosomes to sites related to those observed during thermal mobilization. In contrast, nucleosome mobilization driven by the SWI/SNF and RSC complexes were found to drive nucleosomes towards sites up to 51 bp beyond DNA ends, with little respect for the sites favored during thermal repositioning. The dynamic properties of nucleosomes we describe are likely to influence their role in gene regulation.

    AB - The fundamental subunit of chromatin, the nucleosome, is not a static entity but can move along DNA via either thermal or enzyme-driven movements. Here we have monitored the movements of nucleosomes following deposition at well-defined locations on mouse mammary tumor virus promoter DNA. We found that the sites to which nucleosomes are deposited during chromatin assembly differ from those favored during thermal equilibration. Taking advantage of this, we were able to track the movement of nucleosomes over 156 bp and found that this proceeds via intermediate positions spaced between 46 and 62 bp. The remodeling enzyme ISWI was found to direct the movement of nucleosomes to sites related to those observed during thermal mobilization. In contrast, nucleosome mobilization driven by the SWI/SNF and RSC complexes were found to drive nucleosomes towards sites up to 51 bp beyond DNA ends, with little respect for the sites favored during thermal repositioning. The dynamic properties of nucleosomes we describe are likely to influence their role in gene regulation.

    KW - Chromatin

    KW - Nucleosomes

    KW - Enzyme mobilization

    KW - Thermal mobilization

    U2 - 10.1128/MCB.23.21.7767-7779.2003

    DO - 10.1128/MCB.23.21.7767-7779.2003

    M3 - Article

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    EP - 7779

    JO - Molecular and Cellular Biology

    JF - Molecular and Cellular Biology

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    IS - 21

    ER -