E2F1-dependent methyl cap formation requires RNA pol II phosphorylation

Michael Aregger, Victoria H. Cowling

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    Gene expression is a process integral to cell proliferation. The E2F family of transcription factors upregulates expression of transcripts whose products are essential for cell cycle progression. Here, we report that E2F1 promotes gene expression by an additional mechanism, that is, formation of the methyl cap on RNA pol II transcripts. The methyl cap is required for mRNA maturation, expression and stability. We demonstrate that E2F1 increases RNA pol II phosphorylation, which promotes recruitment of the methyl cap synthetic enzymes. Upregulation of RNA pol II phosphorylation is required for E2F1-dependent methyl cap formation.

    Original languageEnglish
    Pages (from-to)2146-2148
    Number of pages3
    JournalCell Cycle
    Volume11
    Issue number11
    DOIs
    Publication statusPublished - 1 Jun 2012

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