E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis

Leena Ackermann, Michael Schell, Wojciech Pokrzywa, Éva Kevei, Anton Gartner, Björn Schumacher (Lead / Corresponding author), Thorsten Hoppe (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

Multiple protein ubiquitination events at DNA double-strand breaks (DSBs) regulate damage recognition, signaling and repair. It has remained poorly understood how the repair process of DSBs is coordinated with the apoptotic response. Here, we identified the E4 ubiquitin ligase UFD-2 as a mediator of DNA-damage-induced apoptosis in a genetic screen in Caenorhabditis elegans. We found that, after initiation of homologous recombination by RAD-51, UFD-2 forms foci that contain substrate-processivity factors including the ubiquitin-selective segregase CDC-48 (p97), the deubiquitination enzyme ATX-3 (Ataxin-3) and the proteasome. In the absence of UFD-2, RAD-51 foci persist, and DNA damage-induced apoptosis is prevented. In contrast, UFD-2 foci are retained until recombination intermediates are removed by the Holliday-junction-processing enzymes GEN-1, MUS-81 or XPF-1. Formation of UFD-2 foci also requires proapoptotic CEP-1 (p53) signaling. Our findings establish a central role of UFD-2 in the coordination between the DNA-repair process and the apoptotic response.

Original languageEnglish
Pages (from-to)995-1002
Number of pages8
JournalNature Structural & Molecular Biology
Volume23
Issue number11
Early online date26 Sep 2016
DOIs
Publication statusPublished - Nov 2016

Fingerprint

Double-Stranded DNA Breaks
Ubiquitination
Ligases
Ubiquitin
DNA Damage
Cruciform DNA
Apoptosis
Homologous Recombination
Caenorhabditis elegans
Proteasome Endopeptidase Complex
Enzymes
Centers for Disease Control and Prevention (U.S.)
DNA Repair
Genetic Recombination
Proteins
Ataxin-3

Keywords

  • Apoptosis
  • DNA damage response
  • Double-strand DNA breaks
  • Ubiquitin ligases
  • Ubiquitylation

Cite this

Ackermann, Leena ; Schell, Michael ; Pokrzywa, Wojciech ; Kevei, Éva ; Gartner, Anton ; Schumacher, Björn ; Hoppe, Thorsten. / E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis. In: Nature Structural & Molecular Biology. 2016 ; Vol. 23, No. 11. pp. 995-1002.
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abstract = "Multiple protein ubiquitination events at DNA double-strand breaks (DSBs) regulate damage recognition, signaling and repair. It has remained poorly understood how the repair process of DSBs is coordinated with the apoptotic response. Here, we identified the E4 ubiquitin ligase UFD-2 as a mediator of DNA-damage-induced apoptosis in a genetic screen in Caenorhabditis elegans. We found that, after initiation of homologous recombination by RAD-51, UFD-2 forms foci that contain substrate-processivity factors including the ubiquitin-selective segregase CDC-48 (p97), the deubiquitination enzyme ATX-3 (Ataxin-3) and the proteasome. In the absence of UFD-2, RAD-51 foci persist, and DNA damage-induced apoptosis is prevented. In contrast, UFD-2 foci are retained until recombination intermediates are removed by the Holliday-junction-processing enzymes GEN-1, MUS-81 or XPF-1. Formation of UFD-2 foci also requires proapoptotic CEP-1 (p53) signaling. Our findings establish a central role of UFD-2 in the coordination between the DNA-repair process and the apoptotic response.",
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E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis. / Ackermann, Leena; Schell, Michael; Pokrzywa, Wojciech; Kevei, Éva; Gartner, Anton; Schumacher, Björn (Lead / Corresponding author); Hoppe, Thorsten (Lead / Corresponding author).

In: Nature Structural & Molecular Biology, Vol. 23, No. 11, 11.2016, p. 995-1002.

Research output: Contribution to journalArticle

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AU - Ackermann, Leena

AU - Schell, Michael

AU - Pokrzywa, Wojciech

AU - Kevei, Éva

AU - Gartner, Anton

AU - Schumacher, Björn

AU - Hoppe, Thorsten

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N2 - Multiple protein ubiquitination events at DNA double-strand breaks (DSBs) regulate damage recognition, signaling and repair. It has remained poorly understood how the repair process of DSBs is coordinated with the apoptotic response. Here, we identified the E4 ubiquitin ligase UFD-2 as a mediator of DNA-damage-induced apoptosis in a genetic screen in Caenorhabditis elegans. We found that, after initiation of homologous recombination by RAD-51, UFD-2 forms foci that contain substrate-processivity factors including the ubiquitin-selective segregase CDC-48 (p97), the deubiquitination enzyme ATX-3 (Ataxin-3) and the proteasome. In the absence of UFD-2, RAD-51 foci persist, and DNA damage-induced apoptosis is prevented. In contrast, UFD-2 foci are retained until recombination intermediates are removed by the Holliday-junction-processing enzymes GEN-1, MUS-81 or XPF-1. Formation of UFD-2 foci also requires proapoptotic CEP-1 (p53) signaling. Our findings establish a central role of UFD-2 in the coordination between the DNA-repair process and the apoptotic response.

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