Effect of depleted uranium on a soil microcosm fungal community and influence of a plant-ectomycorrhizal association

Marina Fomina, Ji Won Hong, Geoffrey Michael Gadd (Lead / Corresponding author)

Research output: Contribution to journalArticle

Abstract

Fungi are one of the most biogeochemically active components of the soil microbiome, becoming particularly important in metal polluted terrestrial environments. There is scant information on the mycobiota of uranium (U) polluted sites and the effect of metallic depleted uranium (DU) stress on fungal communities in soil has not been reported. The present study aimed to establish the effect of DU contamination on a fungal community in soil using a culture-independent approach, fungal ribosomal intergenic spacer analysis (F-RISA). Experimental soil microcosms also included variants with plants (Pinus silvestris) and P. silvestris/Rhizopogon rubescens ectomycorrhizal associations. Soil contamination with DU resulted in the appearance of RISA bands of the ITS fragments of fungal metagenomic DNA that were characteristic of the genus Mortierella (Mortierellomycotina: Mucoromycota) in pine-free microcosms and for ectomycorrhizal fungi of the genus Scleroderma (Basidiomycota) in microcosms with mycorrhizal pines. The precise taxonomic affinity of the ITS fragments from the band appearing for non-mycorrhizal pines combined with DU remained uncertain, the most likely being related to the subphylum Zoopagomycotina. Thus, soil contamination by thermodynamically unstable metallic depleted uranium can cause a significant change in a soil fungal community under experimental conditions. These changes were also strongly affected by the presence of pine seedlings and their mycorrhizal status which impacted on DU biocorrosion and the release of bioavailable uranium species.

Original languageEnglish
Number of pages8
JournalFungal Biology
Early online date12 Aug 2019
DOIs
Publication statusE-pub ahead of print - 12 Aug 2019

Fingerprint

Uranium
uranium
soil fungi
microcosm
Soil
soil
Pinus
soil pollution
Zoopagomycotina
Scleroderma (fungi)
Pinus sylvestris
Fungi
Fungal DNA
fungus
Rhizopogon
Metagenomics
Basidiomycota
fungi
effect
Microbiota

Keywords

  • F-RISA
  • Fungal communities
  • Fungal stress
  • Geomycology
  • Mycorrhiza

Cite this

@article{45b94dd4d3664ddb97fe310cd4a1a088,
title = "Effect of depleted uranium on a soil microcosm fungal community and influence of a plant-ectomycorrhizal association",
abstract = "Fungi are one of the most biogeochemically active components of the soil microbiome, becoming particularly important in metal polluted terrestrial environments. There is scant information on the mycobiota of uranium (U) polluted sites and the effect of metallic depleted uranium (DU) stress on fungal communities in soil has not been reported. The present study aimed to establish the effect of DU contamination on a fungal community in soil using a culture-independent approach, fungal ribosomal intergenic spacer analysis (F-RISA). Experimental soil microcosms also included variants with plants (Pinus silvestris) and P. silvestris/Rhizopogon rubescens ectomycorrhizal associations. Soil contamination with DU resulted in the appearance of RISA bands of the ITS fragments of fungal metagenomic DNA that were characteristic of the genus Mortierella (Mortierellomycotina: Mucoromycota) in pine-free microcosms and for ectomycorrhizal fungi of the genus Scleroderma (Basidiomycota) in microcosms with mycorrhizal pines. The precise taxonomic affinity of the ITS fragments from the band appearing for non-mycorrhizal pines combined with DU remained uncertain, the most likely being related to the subphylum Zoopagomycotina. Thus, soil contamination by thermodynamically unstable metallic depleted uranium can cause a significant change in a soil fungal community under experimental conditions. These changes were also strongly affected by the presence of pine seedlings and their mycorrhizal status which impacted on DU biocorrosion and the release of bioavailable uranium species.",
keywords = "F-RISA, Fungal communities, Fungal stress, Geomycology, Mycorrhiza",
author = "Marina Fomina and Hong, {Ji Won} and Gadd, {Geoffrey Michael}",
note = "Funding: MOD/NERC DU Programme (Grant NE/C506799/1)",
year = "2019",
month = "8",
day = "12",
doi = "10.1016/j.funbio.2019.08.001",
language = "English",
journal = "Fungal Biology",
issn = "1878-6146",
publisher = "Elsevier",

}

TY - JOUR

T1 - Effect of depleted uranium on a soil microcosm fungal community and influence of a plant-ectomycorrhizal association

AU - Fomina, Marina

AU - Hong, Ji Won

AU - Gadd, Geoffrey Michael

N1 - Funding: MOD/NERC DU Programme (Grant NE/C506799/1)

PY - 2019/8/12

Y1 - 2019/8/12

N2 - Fungi are one of the most biogeochemically active components of the soil microbiome, becoming particularly important in metal polluted terrestrial environments. There is scant information on the mycobiota of uranium (U) polluted sites and the effect of metallic depleted uranium (DU) stress on fungal communities in soil has not been reported. The present study aimed to establish the effect of DU contamination on a fungal community in soil using a culture-independent approach, fungal ribosomal intergenic spacer analysis (F-RISA). Experimental soil microcosms also included variants with plants (Pinus silvestris) and P. silvestris/Rhizopogon rubescens ectomycorrhizal associations. Soil contamination with DU resulted in the appearance of RISA bands of the ITS fragments of fungal metagenomic DNA that were characteristic of the genus Mortierella (Mortierellomycotina: Mucoromycota) in pine-free microcosms and for ectomycorrhizal fungi of the genus Scleroderma (Basidiomycota) in microcosms with mycorrhizal pines. The precise taxonomic affinity of the ITS fragments from the band appearing for non-mycorrhizal pines combined with DU remained uncertain, the most likely being related to the subphylum Zoopagomycotina. Thus, soil contamination by thermodynamically unstable metallic depleted uranium can cause a significant change in a soil fungal community under experimental conditions. These changes were also strongly affected by the presence of pine seedlings and their mycorrhizal status which impacted on DU biocorrosion and the release of bioavailable uranium species.

AB - Fungi are one of the most biogeochemically active components of the soil microbiome, becoming particularly important in metal polluted terrestrial environments. There is scant information on the mycobiota of uranium (U) polluted sites and the effect of metallic depleted uranium (DU) stress on fungal communities in soil has not been reported. The present study aimed to establish the effect of DU contamination on a fungal community in soil using a culture-independent approach, fungal ribosomal intergenic spacer analysis (F-RISA). Experimental soil microcosms also included variants with plants (Pinus silvestris) and P. silvestris/Rhizopogon rubescens ectomycorrhizal associations. Soil contamination with DU resulted in the appearance of RISA bands of the ITS fragments of fungal metagenomic DNA that were characteristic of the genus Mortierella (Mortierellomycotina: Mucoromycota) in pine-free microcosms and for ectomycorrhizal fungi of the genus Scleroderma (Basidiomycota) in microcosms with mycorrhizal pines. The precise taxonomic affinity of the ITS fragments from the band appearing for non-mycorrhizal pines combined with DU remained uncertain, the most likely being related to the subphylum Zoopagomycotina. Thus, soil contamination by thermodynamically unstable metallic depleted uranium can cause a significant change in a soil fungal community under experimental conditions. These changes were also strongly affected by the presence of pine seedlings and their mycorrhizal status which impacted on DU biocorrosion and the release of bioavailable uranium species.

KW - F-RISA

KW - Fungal communities

KW - Fungal stress

KW - Geomycology

KW - Mycorrhiza

UR - http://www.scopus.com/inward/record.url?scp=85071401569&partnerID=8YFLogxK

U2 - 10.1016/j.funbio.2019.08.001

DO - 10.1016/j.funbio.2019.08.001

M3 - Article

JO - Fungal Biology

JF - Fungal Biology

SN - 1878-6146

ER -