Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids

Evangelia Stavridou, Nikoleta A. Τzioutziou, Panagiotis Madesis, Nikolaos E. Labrou, Irini Nianiou-Obeidat

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Abstract

The current study aimed to produce rootstock material through micropropagation by developing efficient regeneration and Agrobacterium-mediated transformation protocols for three high quality commercial tomato hybrids (Felina, Siena and Don Jose) to overexpress the GmGSTU4 gene from Glycine max L. previously shown to enhance antioxidant activity. We investigated the plant growth regulators zeatin (Z) and 3-idoleacetic acid (IAA) to determine their best combination for an efficient regeneration protocol for each hybrid. The highest regeneration efficiency was observed in Felina (94.4%) with 1.0 mg/l Z and 0.1 mg/l IAA. In contrast, Don Jose (92.5%) and Siena (83.3%) performed better with 0.5 mg/l Z and 0.1 mg/l IAA. The three hybrids did not differ in micropropagation index, however, Felina showed the highest number of in vitro rooted and in vivo acclimatized plants. Factors such as the age of explant, days in pre- and co-culture and the concentrations of acetosyringone and thiamine on Agrobacterium-mediated genetic transformation were assessed. The transformation indices were 37.04% for the Felina, 13.8% for Siena and 8.33% for Don Jose. We conclude that targeted genotype-specific regeneration protocols will provide an efficient and cost effective genetic transformation system for rootstock production and further incorporation into micropropagation and transgrafting systems.

Original languageEnglish
Pages (from-to)120-127
Number of pages8
JournalCzech Journal of Genetics and Plant Breeding
Volume55
Issue number3
Early online date18 Mar 2019
DOIs
Publication statusPublished - 2019

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Lycopersicon esculentum
micropropagation
Regeneration
tomatoes
genetic transformation
Genetic Transformation
Agrobacterium
rootstocks
acids
Acids
acetosyringone
Zeatin
zeatin
coculture
thiamin
plant growth substances
Plant Growth Regulators
Glycine max
Thiamine
explants

Keywords

  • Agrobacterium-mediated transformation
  • Glutathione-S-transferases
  • Growth regulators
  • Micropropagation
  • Shoot regeneration
  • Solanum lycopersicum

Cite this

Stavridou, E., Τzioutziou, N. A., Madesis, P., Labrou, N. E., & Nianiou-Obeidat, I. (2019). Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids. Czech Journal of Genetics and Plant Breeding, 55(3), 120-127. https://doi.org/10.17221/61/2018-CJGPB
Stavridou, Evangelia ; Τzioutziou, Nikoleta A. ; Madesis, Panagiotis ; Labrou, Nikolaos E. ; Nianiou-Obeidat, Irini. / Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids. In: Czech Journal of Genetics and Plant Breeding. 2019 ; Vol. 55, No. 3. pp. 120-127.
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Stavridou, E, Τzioutziou, NA, Madesis, P, Labrou, NE & Nianiou-Obeidat, I 2019, 'Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids', Czech Journal of Genetics and Plant Breeding, vol. 55, no. 3, pp. 120-127. https://doi.org/10.17221/61/2018-CJGPB

Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids. / Stavridou, Evangelia; Τzioutziou, Nikoleta A.; Madesis, Panagiotis; Labrou, Nikolaos E.; Nianiou-Obeidat, Irini.

In: Czech Journal of Genetics and Plant Breeding, Vol. 55, No. 3, 2019, p. 120-127.

Research output: Contribution to journalArticle

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T1 - Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids

AU - Stavridou, Evangelia

AU - Τzioutziou, Nikoleta A.

AU - Madesis, Panagiotis

AU - Labrou, Nikolaos E.

AU - Nianiou-Obeidat, Irini

PY - 2019

Y1 - 2019

N2 - The current study aimed to produce rootstock material through micropropagation by developing efficient regeneration and Agrobacterium-mediated transformation protocols for three high quality commercial tomato hybrids (Felina, Siena and Don Jose) to overexpress the GmGSTU4 gene from Glycine max L. previously shown to enhance antioxidant activity. We investigated the plant growth regulators zeatin (Z) and 3-idoleacetic acid (IAA) to determine their best combination for an efficient regeneration protocol for each hybrid. The highest regeneration efficiency was observed in Felina (94.4%) with 1.0 mg/l Z and 0.1 mg/l IAA. In contrast, Don Jose (92.5%) and Siena (83.3%) performed better with 0.5 mg/l Z and 0.1 mg/l IAA. The three hybrids did not differ in micropropagation index, however, Felina showed the highest number of in vitro rooted and in vivo acclimatized plants. Factors such as the age of explant, days in pre- and co-culture and the concentrations of acetosyringone and thiamine on Agrobacterium-mediated genetic transformation were assessed. The transformation indices were 37.04% for the Felina, 13.8% for Siena and 8.33% for Don Jose. We conclude that targeted genotype-specific regeneration protocols will provide an efficient and cost effective genetic transformation system for rootstock production and further incorporation into micropropagation and transgrafting systems.

AB - The current study aimed to produce rootstock material through micropropagation by developing efficient regeneration and Agrobacterium-mediated transformation protocols for three high quality commercial tomato hybrids (Felina, Siena and Don Jose) to overexpress the GmGSTU4 gene from Glycine max L. previously shown to enhance antioxidant activity. We investigated the plant growth regulators zeatin (Z) and 3-idoleacetic acid (IAA) to determine their best combination for an efficient regeneration protocol for each hybrid. The highest regeneration efficiency was observed in Felina (94.4%) with 1.0 mg/l Z and 0.1 mg/l IAA. In contrast, Don Jose (92.5%) and Siena (83.3%) performed better with 0.5 mg/l Z and 0.1 mg/l IAA. The three hybrids did not differ in micropropagation index, however, Felina showed the highest number of in vitro rooted and in vivo acclimatized plants. Factors such as the age of explant, days in pre- and co-culture and the concentrations of acetosyringone and thiamine on Agrobacterium-mediated genetic transformation were assessed. The transformation indices were 37.04% for the Felina, 13.8% for Siena and 8.33% for Don Jose. We conclude that targeted genotype-specific regeneration protocols will provide an efficient and cost effective genetic transformation system for rootstock production and further incorporation into micropropagation and transgrafting systems.

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KW - Glutathione-S-transferases

KW - Growth regulators

KW - Micropropagation

KW - Shoot regeneration

KW - Solanum lycopersicum

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M3 - Article

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Stavridou E, Τzioutziou NA, Madesis P, Labrou NE, Nianiou-Obeidat I. Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids. Czech Journal of Genetics and Plant Breeding. 2019;55(3):120-127. https://doi.org/10.17221/61/2018-CJGPB