Abstract
The current study aimed to produce rootstock material through micropropagation by developing efficient regeneration and Agrobacterium-mediated transformation protocols for three high quality commercial tomato hybrids (Felina, Siena and Don Jose) to overexpress the GmGSTU4 gene from Glycine max L. previously shown to enhance antioxidant activity. We investigated the plant growth regulators zeatin (Z) and 3-idoleacetic acid (IAA) to determine their best combination for an efficient regeneration protocol for each hybrid. The highest regeneration efficiency was observed in Felina (94.4%) with 1.0 mg/l Z and 0.1 mg/l IAA. In contrast, Don Jose (92.5%) and Siena (83.3%) performed better with 0.5 mg/l Z and 0.1 mg/l IAA. The three hybrids did not differ in micropropagation index, however, Felina showed the highest number of in vitro rooted and in vivo acclimatized plants. Factors such as the age of explant, days in pre- and co-culture and the concentrations of acetosyringone and thiamine on Agrobacterium-mediated genetic transformation were assessed. The transformation indices were 37.04% for the Felina, 13.8% for Siena and 8.33% for Don Jose. We conclude that targeted genotype-specific regeneration protocols will provide an efficient and cost effective genetic transformation system for rootstock production and further incorporation into micropropagation and transgrafting systems.
| Original language | English |
|---|---|
| Pages (from-to) | 120-127 |
| Number of pages | 8 |
| Journal | Czech Journal of Genetics and Plant Breeding |
| Volume | 55 |
| Issue number | 3 |
| Early online date | 18 Mar 2019 |
| DOIs | |
| Publication status | Published - 2019 |
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Keywords
- Agrobacterium-mediated transformation
- Glutathione-S-transferases
- Growth regulators
- Micropropagation
- Shoot regeneration
- Solanum lycopersicum
Cite this
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Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids. / Stavridou, Evangelia; Τzioutziou, Nikoleta A.; Madesis, Panagiotis; Labrou, Nikolaos E.; Nianiou-Obeidat, Irini.
In: Czech Journal of Genetics and Plant Breeding, Vol. 55, No. 3, 2019, p. 120-127.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effect of different factors on regeneration and transformation efficiency of tomato (Lycopersicum esculentum) hybrids
AU - Stavridou, Evangelia
AU - Τzioutziou, Nikoleta A.
AU - Madesis, Panagiotis
AU - Labrou, Nikolaos E.
AU - Nianiou-Obeidat, Irini
PY - 2019
Y1 - 2019
N2 - The current study aimed to produce rootstock material through micropropagation by developing efficient regeneration and Agrobacterium-mediated transformation protocols for three high quality commercial tomato hybrids (Felina, Siena and Don Jose) to overexpress the GmGSTU4 gene from Glycine max L. previously shown to enhance antioxidant activity. We investigated the plant growth regulators zeatin (Z) and 3-idoleacetic acid (IAA) to determine their best combination for an efficient regeneration protocol for each hybrid. The highest regeneration efficiency was observed in Felina (94.4%) with 1.0 mg/l Z and 0.1 mg/l IAA. In contrast, Don Jose (92.5%) and Siena (83.3%) performed better with 0.5 mg/l Z and 0.1 mg/l IAA. The three hybrids did not differ in micropropagation index, however, Felina showed the highest number of in vitro rooted and in vivo acclimatized plants. Factors such as the age of explant, days in pre- and co-culture and the concentrations of acetosyringone and thiamine on Agrobacterium-mediated genetic transformation were assessed. The transformation indices were 37.04% for the Felina, 13.8% for Siena and 8.33% for Don Jose. We conclude that targeted genotype-specific regeneration protocols will provide an efficient and cost effective genetic transformation system for rootstock production and further incorporation into micropropagation and transgrafting systems.
AB - The current study aimed to produce rootstock material through micropropagation by developing efficient regeneration and Agrobacterium-mediated transformation protocols for three high quality commercial tomato hybrids (Felina, Siena and Don Jose) to overexpress the GmGSTU4 gene from Glycine max L. previously shown to enhance antioxidant activity. We investigated the plant growth regulators zeatin (Z) and 3-idoleacetic acid (IAA) to determine their best combination for an efficient regeneration protocol for each hybrid. The highest regeneration efficiency was observed in Felina (94.4%) with 1.0 mg/l Z and 0.1 mg/l IAA. In contrast, Don Jose (92.5%) and Siena (83.3%) performed better with 0.5 mg/l Z and 0.1 mg/l IAA. The three hybrids did not differ in micropropagation index, however, Felina showed the highest number of in vitro rooted and in vivo acclimatized plants. Factors such as the age of explant, days in pre- and co-culture and the concentrations of acetosyringone and thiamine on Agrobacterium-mediated genetic transformation were assessed. The transformation indices were 37.04% for the Felina, 13.8% for Siena and 8.33% for Don Jose. We conclude that targeted genotype-specific regeneration protocols will provide an efficient and cost effective genetic transformation system for rootstock production and further incorporation into micropropagation and transgrafting systems.
KW - Agrobacterium-mediated transformation
KW - Glutathione-S-transferases
KW - Growth regulators
KW - Micropropagation
KW - Shoot regeneration
KW - Solanum lycopersicum
UR - http://www.scopus.com/inward/record.url?scp=85071186579&partnerID=8YFLogxK
U2 - 10.17221/61/2018-CJGPB
DO - 10.17221/61/2018-CJGPB
M3 - Article
VL - 55
SP - 120
EP - 127
IS - 3
ER -