Effect of high physiological levels of factor VIII:C and factor V on rate of generation of thrombin activity in vitro

S. H. Ibbotson, G. M. Tate, J. A. Davies

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Increased plasma FVIII:C concentrations occur in several conditions, including diabetes, but whether this leads to clinically relevant hypercoagulability is uncertain. Accordingly, we investigated the effects of increasing FVIII:C levels on coagulation in vitro using a computer-assisted measurement of thrombin activity. Defibrinated plasma was activated with kaolin, thrombin activity measured using the chromogenic substrate S2238 and time to generate 50% maximal thrombin activity (T50) recorded in seconds. Increasing FVIII:C levels from 100 to 350% significantly reduced T50 (mean +/- SD) from 91 +/- 3 to 64 +/- 6.2 s (P <0.001, n = 6), and T50 correlated inversely with FVIII:C (r = -0.884, P <0.001, n = 36). Increasing FV concentrations resulted in an additive effect with high FVIII:C levels on the rate of thrombin generation. The results showed that increasing plasma FVIII:C and FV concentrations accelerate rate of generation of thrombin activity independently, and in an additive manner.
    Original languageEnglish
    Pages (from-to)415-419
    Number of pages5
    JournalBlood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
    Volume4
    Issue number3
    Publication statusPublished - Jun 1993

    Keywords

    • Thrombin
    • Stimulation, Chemical
    • Factor V
    • Blood Coagulation
    • Microcomputers
    • Kinetics
    • Humans
    • Dipeptides
    • Kaolin
    • Factor VIII

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