Effect of human cervical mucus on human sperm motion and hyperactivation in vitro

J. J. Zhu, C. L. R. Barratt, I. D. Cooke

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


The aim of our experiment was to examine the effect of exposure to human cervical mucus on quantitative sperm motility with specific reference to hyperactivated sperm motility. Human spermatozoa were allowed to penetrate cervical mucus for 20 min before swimming into Earle's balanced salt solution tissue culture medium for 25 min. The sperm motion characteristics were compared to those which had been obtained from a direct swim-up for 45 min. Spermatozoa treated with mucus were more 'active' than the control group. Multivariate statistical analysis indicated that cervical mucus promotes hyperactivated motility and that sperm sub-populations exposed to cervical mucus are very heterogeneous, as indicated by the numbers and motility characteristics of spermatozoa.

Original languageEnglish
Pages (from-to)1402-1406
Number of pages5
JournalHuman Reproduction
Issue number10
Publication statusPublished - Nov 1992


  • Cervix Mucus/physiology
  • Cluster Analysis
  • Computers
  • Female
  • Humans
  • In Vitro Techniques
  • Male
  • Multivariate Analysis
  • Sperm Motility


Dive into the research topics of 'Effect of human cervical mucus on human sperm motion and hyperactivation in vitro'. Together they form a unique fingerprint.

Cite this