Effect of methylation of adenine N6 on kink turn structure depends on location

Saira Ashraf, Lin Huang, David Lilley (Lead / Corresponding author)

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    8 Citations (Scopus)
    134 Downloads (Pure)


    N6-methyladenine is the most common covalent modification in cellular RNA species, with demonstrated functional consequences. At the molecular level this methylation could alter local RNA structure, and/or modulate the binding of specific proteins. We have previously shown that trans-Hoogsteen-sugar (sheared) A:G base pairs can be completely disrupted by methylation, and that this occurs in a sub-set of human box C/D k-turn structures. In this work we have investigated to what extent sequence context affects the severity with which inclusion of N6-methyladenine into different A:G base pairs of a standard k-turn affects RNA folding and L7Ae protein binding. We find that local sequence has a major influence, ranging from complete absence of folding and protein binding to a relatively mild effect. We have determined the crystal structure of one of these species both free and protein-bound, showing the environment of the methyl group and the way the modification is accommodated into the k-turn structure.
    Original languageEnglish
    Pages (from-to)1377-1385
    Number of pages9
    JournalRNA Biology
    Issue number10
    Early online date24 Jun 2019
    Publication statusPublished - 2019


    • RNA structure
    • RNA methylation
    • epigenetic modification
    • N6-methyladenine
    • kink-turn
    • X-ray crysallography

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology


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