Effect of nucleosides and nucleotides and the relationship between cellular adenosine 3′:5′-cyclic monophosphate (cyclic AMP) and germ tube formation in Candida albicans

F. T. Sabie, G. M. Gadd

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    53 Citations (Scopus)

    Abstract

    A yeast-mycelium (Y-M) transition in Candida albicans was induced by exogenous yeast extract, adenosine, adenosine 5′-monophosphate (AMP), adenosine 5′-diphosphate (ADP), adenosine 3′:5′ cyclic monophosphate (cAMP) and its analogue N6, O2′-dibutyryl adenosine 3′:5′-cyclic monophosphate (dbcAMP) in defined liquid medium at 25°C. Adenosine 5′-triphosphate (ATP) was found to delay germ tube formation in yeast cells, whereas the cAMP phosphodiesterase inhibitors, theophylline and caffeine, induced a Y-M transition. Intracellular and extracellular cyclic AMP levels increased during the yeast-mycelium transition and maximum levels of intracellular cyclic AMP coincided with maximum germ tube formation. Of the many inducers and inhibitors of germ tube and mycelium formation in C. albicans tested, including incubation at 37°C or in the presence of 1.5mM CaCl2, the calmodulin inhibitor calmidazolium (R24571) added together with CaCl2 induced the highest intra- and extracellular cyclic AMP levels. These results confirm the involvement of cyclic AMP in the yeast-mycelium transition of C. albicans.

    Original languageEnglish
    Pages (from-to)147-156
    Number of pages10
    JournalMycopathologia
    Volume119
    Issue number3
    DOIs
    Publication statusPublished - Sep 1992

    Keywords

    • adenosine 3′5′-cyclic monophosphate
    • Candida albicans
    • cyclic AMP
    • dimorphism
    • yeast-mycelium transition

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