Effect of nutritional imbalances on cytochrome P-450 isozymes in rat liver

Z Amelizad, J F Narbonne (Lead / Corresponding author), C R Wolf, L W Robertson, F Oesch

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Male Sprague-Dawley rats were fed for six weeks either a control diet containing 22% casein (C) and 5% fat (F) or a low-protein diet (6% C, 5% F) or high-lipid diet (30% C, 30% F). A group of rats received a control diet containing 50 ppm of Phenoclor DP6. Three major forms of cytochrome P-450, UT 50, BP 3a and MC 2 were purified from livers of DP6-fed rats and only two forms, UT 50 and PB 3a, were purified from control and dietary groups. The amino acid composition and the catalytic activities towards all substrates tested were only significantly modified in the purified UT 50 P-450 isozyme from rats fed the low-protein diet. The N-terminal sequence analysis shows that cytochrome P-450 UT 50 (from control group) and UT 501 (from low-protein group) are two distinct proteins.

Original languageEnglish
Pages (from-to)3245-9
Number of pages5
JournalBiochemical Pharmacology
Issue number17
Publication statusPublished - 1 Sept 1988


  • Amino Acids/analysis
  • Animal Nutritional Physiological Phenomena
  • Animals
  • Cytochrome P-450 Enzyme System/metabolism
  • Enzyme Induction
  • Immunologic Techniques
  • Isoenzymes/metabolism
  • Microsomes, Liver/enzymology
  • Rats


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