Effect of reduced irradiance on 13C uptake, gene expression and protein activity of the seagrass Zostera muelleri

Mikael Kim, Mathieu Pernice (Lead / Corresponding author), Alexander Watson-Lazowski, Paul Guagliardo, Matt R. Kilburn, Anthony W. D. Larkum, John A. Raven, Peter J. Ralph

    Research output: Contribution to journalArticle

    Abstract

    Photosynthesis in the seagrass Zostera muelleri remains poorly understood. We investigated the effect of reduced irradiance on the incorporation of 13C, gene expression of photosynthetic, photorespiratory and intermediates recycling genes as well as the enzymatic content and activity of Rubisco and PEPC within Z. muelleri. Following 48 h of reduced irradiance, we found that i) there was a ∼7 fold reduction in 13C incorporation in above ground tissue, ii) a significant down regulation of photosynthetic, photorespiratory and intermediates recycling genes and iii) no significant difference in enzyme activity and content. We propose that Z. muelleri is able to alter its physiology in order to reduce the amount of C lost through photorespiration to compensate for the reduced carbon assimilation as a result of reduced irradiance. In addition, the first estimated rate constant (Kcat) and maximum rates of carboxylation (Vcmax) of Rubisco is reported for the first time for Z. muelleri.

    Original languageEnglish
    Pages (from-to)80-89
    Number of pages10
    JournalMarine Environmental Research
    Volume149
    Early online date6 Jun 2019
    DOIs
    Publication statusPublished - 1 Aug 2019

    Fingerprint

    Zostera
    ribulose-bisphosphate carboxylase
    seagrass
    Gene expression
    recycling
    gene expression
    irradiance
    Recycling
    Genes
    Carboxylation
    Proteins
    uptake mechanisms
    protein
    photorespiration
    Photosynthesis
    carboxylation
    Physiology
    Enzyme activity
    Rate constants
    genes

    Keywords

    • Australia
    • Genetics
    • Nanostring
    • Photorespiration
    • Photosynthesis
    • Seagrass
    • Stable isotope
    • Zostera muelleri

    Cite this

    Kim, M., Pernice, M., Watson-Lazowski, A., Guagliardo, P., Kilburn, M. R., Larkum, A. W. D., ... Ralph, P. J. (2019). Effect of reduced irradiance on 13C uptake, gene expression and protein activity of the seagrass Zostera muelleri. Marine Environmental Research, 149, 80-89. https://doi.org/10.1016/j.marenvres.2019.06.004
    Kim, Mikael ; Pernice, Mathieu ; Watson-Lazowski, Alexander ; Guagliardo, Paul ; Kilburn, Matt R. ; Larkum, Anthony W. D. ; Raven, John A. ; Ralph, Peter J. / Effect of reduced irradiance on 13C uptake, gene expression and protein activity of the seagrass Zostera muelleri. In: Marine Environmental Research. 2019 ; Vol. 149. pp. 80-89.
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    abstract = "Photosynthesis in the seagrass Zostera muelleri remains poorly understood. We investigated the effect of reduced irradiance on the incorporation of 13C, gene expression of photosynthetic, photorespiratory and intermediates recycling genes as well as the enzymatic content and activity of Rubisco and PEPC within Z. muelleri. Following 48 h of reduced irradiance, we found that i) there was a ∼7 fold reduction in 13C incorporation in above ground tissue, ii) a significant down regulation of photosynthetic, photorespiratory and intermediates recycling genes and iii) no significant difference in enzyme activity and content. We propose that Z. muelleri is able to alter its physiology in order to reduce the amount of C lost through photorespiration to compensate for the reduced carbon assimilation as a result of reduced irradiance. In addition, the first estimated rate constant (Kcat) and maximum rates of carboxylation (Vcmax) of Rubisco is reported for the first time for Z. muelleri.",
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    Kim, M, Pernice, M, Watson-Lazowski, A, Guagliardo, P, Kilburn, MR, Larkum, AWD, Raven, JA & Ralph, PJ 2019, 'Effect of reduced irradiance on 13C uptake, gene expression and protein activity of the seagrass Zostera muelleri', Marine Environmental Research, vol. 149, pp. 80-89. https://doi.org/10.1016/j.marenvres.2019.06.004

    Effect of reduced irradiance on 13C uptake, gene expression and protein activity of the seagrass Zostera muelleri. / Kim, Mikael; Pernice, Mathieu (Lead / Corresponding author); Watson-Lazowski, Alexander; Guagliardo, Paul; Kilburn, Matt R.; Larkum, Anthony W. D.; Raven, John A.; Ralph, Peter J.

    In: Marine Environmental Research, Vol. 149, 01.08.2019, p. 80-89.

    Research output: Contribution to journalArticle

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