Effect of salinity on the synthesis of ribulose-1,5-bisphosphate carboxylase/oxygenase in barley leaves

T. S. Miteva, N. Zh. Zhelev, L. P. Popova

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)

    Abstract

    Ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) (RubisCO) was quantified in 7-day-old leaves of barley (Hordeum vulgare L., var. Alfa) treated with NaCl using polyacrylamide gel electrophoresis. The treated plants showed a decrease in the level of RubisCO. In the presence of 100 mM NaCl the level of total soluble protein on a fresh weight basis was about 64% of the control, whereas the level of RubisCO was only about 20% that of control plants. Salinity induced marked quantitative and qualitative changes in polypeptide profiles of soluble leaf proteins. Enhanced levels of 76, 60, 47, 43 and 30 kD polypeptides and reduced levels of 55 and 15 kD polypeptides were observed in NaCl-treated plants. The appearance of one new polypeptide of 26-25 kD was found in NaCl-treated samples. The influence of salinity on the synthesis of RubisCO was followed by in vivo labeling with a 14C-amino acid mixture (14C-AAM). The results demonstrate that salinity inhibits the synthesis of total soluble protein with a more pronounced inhibition of synthesis of RubisCO.

    Original languageEnglish
    Pages (from-to)46-51
    Number of pages6
    JournalJournal of Plant Physiology
    Volume140
    Issue number1
    DOIs
    Publication statusPublished - May 1992

    Keywords

    • C-AAM
    • C-amino acid mixture
    • Hordeum vulgare (L.)
    • large subunit of RubisCO
    • LSU
    • NaCl-salinity
    • NaCl-stress-induced polypeptides
    • PEPCase
    • phosphoenolpyruvate carboxylase
    • protein synthesis
    • ribulose-1,5-bisphosphate carboxylase/oxygenase
    • RubisCO
    • RubisCO synthesis
    • small subunit of RubisCO
    • SSU
    • TCA
    • trichloroacetic acid

    ASJC Scopus subject areas

    • Physiology
    • Agronomy and Crop Science
    • Plant Science

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