Effector PiAVR2 targets BSL family members which act as susceptibility factors to suppress immunity

Dionne Turnbull, Haixia Wang, Susan Breen, Marek Malec, Shaista Naqvi, Lina Yang, Lydia Welsh, Piers Hemsley, Zhendong Tian, Frédéric Brunner, Eleanor M. Gilroy, Paul Birch (Lead / Corresponding author)

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Abstract

To be successful plant pathogens, microbes use ‘effector proteins’ to manipulate host functions to their benefit. Identifying host targets of effector proteins, and characterising their role in the infection process, allows us to better understand plant-pathogen interactions and the plant immune system. Yeast-2-hybrid and co-immunoprecipitation were used to demonstrate that Phytophthora infestans effector PiAVR2 interacts with all three BSU1-like (BSL) family members from Solanum tuberosum. Transient expression of BSL1, 2 and 3 enhanced P. infestans leaf infection. BSL1 and BSL3 suppressed INF1 elicitin-triggered cell death, showing that they negatively regulate immunity. Virus-induced gene silencing studies revealed that BSL2 and 3 are required for BSL1 stability, and show that basal levels of immunity are increased in BSL-silenced plants. Immune suppression by BSL family members is dependent on the brassinosteroid-responsive host transcription factor CHL1. P. infestans effector PiAVR2 targets all three BSL family members in the crop plant S. tuberosum. These phosphatases, known for their role in growth-promoting brassinosteroid signalling, all support P. infestans virulence, and thus can be regarded as susceptibility factors in late blight infection.
Original languageEnglish
Pages (from-to)571-581
Number of pages11
JournalPlant Physiology
Volume180
Issue number1
Early online date19 Feb 2019
DOIs
Publication statusPublished - 1 May 2019

Keywords

  • Plant immunity
  • effector-triggered susceptibility
  • plant disease
  • Late blight
  • virulence
  • Plant pathogens
  • Pathogenicity

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