Effects of a grapeseed procyanidin extract (GSPE) on insulin resistance

Gemma Montagut Pino, Cinta Bladé, Mayte Blay, Juan Fernández-Larrea, Gerard Pujadas, M. Josepa Salvadó, Lluis Arola, Monserrat Pinent, Anna Ardévol

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    Abstract

    Flavonoids are beneficial compounds against risk factors for metabolic syndrome, but their effects and the mechanisms on glucose homeostasis modulation are not well defined. In the present study, we first checked the efficacy of grapeseed procyanidin extract (GSPE) for stimulating glucose uptake in insulin-resistant 3T3-L1 adipocytes. Results show that when resistance is induced with chronic insulin treatment, GSPE maintain a higher stimulating capacity than insulin. In contrast, when dexamethasone is used as the resistance-inducing agent, GSPE is less effective. Next we evaluated how effective different GSPE treatments are at improving glucose metabolism in hyperinsulinemic animals (fed a cafeteria diet). GSPE reduced plasma insulin levels. The lower dose (25 mg GSPE/kg body weight per day) administered for 30 days improved the HOmeostasis Model Assessment-insulin resistance index. This was accompanied by down-regulation of Pparg2, Glut4 and Irs1 in mesenteric white adipose tissue. Similarly, a chronic GSPE treatment of insulin-resistant 3T3-L1 adipocytes down-regulated the mRNA levels of those adipocyte markers, although cells were still able to respond to the acute stimulation of glucose uptake.In summary, 25 mg/kg body weight per day of GSPE has a positive long-term effect on glucose homeostasis, and GSPE could be targeted at adipose tissue, where it might directly stimulate glucose uptake. This work also highlights the need to carefully consider the bioactive dose, since a higher dose does not necessarily correlate to a greater positive effect.
    Original languageEnglish
    Pages (from-to)961-967
    Number of pages7
    JournalJournal of Nutritional Biochemistry
    Volume21
    Issue number10
    DOIs
    Publication statusPublished - Oct 2010

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    Insulin Resistance
    Insulin
    Glucose
    Adipocytes
    Homeostasis
    Body Weight
    Tissue
    procyanidin
    White Adipose Tissue
    Nutrition
    Flavonoids
    Metabolism
    Dexamethasone
    Adipose Tissue
    Animals
    Therapeutics
    Down-Regulation
    Modulation
    Diet
    Plasmas

    Cite this

    Montagut Pino, G., Bladé, C., Blay, M., Fernández-Larrea, J., Pujadas, G., Salvadó, M. J., ... Ardévol, A. (2010). Effects of a grapeseed procyanidin extract (GSPE) on insulin resistance. Journal of Nutritional Biochemistry, 21(10), 961-967. https://doi.org/10.1016/j.jnutbio.2009.08.001
    Montagut Pino, Gemma ; Bladé, Cinta ; Blay, Mayte ; Fernández-Larrea, Juan ; Pujadas, Gerard ; Salvadó, M. Josepa ; Arola, Lluis ; Pinent, Monserrat ; Ardévol, Anna. / Effects of a grapeseed procyanidin extract (GSPE) on insulin resistance. In: Journal of Nutritional Biochemistry. 2010 ; Vol. 21, No. 10. pp. 961-967.
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    abstract = "Flavonoids are beneficial compounds against risk factors for metabolic syndrome, but their effects and the mechanisms on glucose homeostasis modulation are not well defined. In the present study, we first checked the efficacy of grapeseed procyanidin extract (GSPE) for stimulating glucose uptake in insulin-resistant 3T3-L1 adipocytes. Results show that when resistance is induced with chronic insulin treatment, GSPE maintain a higher stimulating capacity than insulin. In contrast, when dexamethasone is used as the resistance-inducing agent, GSPE is less effective. Next we evaluated how effective different GSPE treatments are at improving glucose metabolism in hyperinsulinemic animals (fed a cafeteria diet). GSPE reduced plasma insulin levels. The lower dose (25 mg GSPE/kg body weight per day) administered for 30 days improved the HOmeostasis Model Assessment-insulin resistance index. This was accompanied by down-regulation of Pparg2, Glut4 and Irs1 in mesenteric white adipose tissue. Similarly, a chronic GSPE treatment of insulin-resistant 3T3-L1 adipocytes down-regulated the mRNA levels of those adipocyte markers, although cells were still able to respond to the acute stimulation of glucose uptake.In summary, 25 mg/kg body weight per day of GSPE has a positive long-term effect on glucose homeostasis, and GSPE could be targeted at adipose tissue, where it might directly stimulate glucose uptake. This work also highlights the need to carefully consider the bioactive dose, since a higher dose does not necessarily correlate to a greater positive effect.",
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    Montagut Pino, G, Bladé, C, Blay, M, Fernández-Larrea, J, Pujadas, G, Salvadó, MJ, Arola, L, Pinent, M & Ardévol, A 2010, 'Effects of a grapeseed procyanidin extract (GSPE) on insulin resistance', Journal of Nutritional Biochemistry, vol. 21, no. 10, pp. 961-967. https://doi.org/10.1016/j.jnutbio.2009.08.001

    Effects of a grapeseed procyanidin extract (GSPE) on insulin resistance. / Montagut Pino, Gemma; Bladé, Cinta; Blay, Mayte; Fernández-Larrea, Juan; Pujadas, Gerard; Salvadó, M. Josepa; Arola, Lluis; Pinent, Monserrat; Ardévol, Anna.

    In: Journal of Nutritional Biochemistry, Vol. 21, No. 10, 10.2010, p. 961-967.

    Research output: Contribution to journalArticle

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    AU - Montagut Pino, Gemma

    AU - Bladé, Cinta

    AU - Blay, Mayte

    AU - Fernández-Larrea, Juan

    AU - Pujadas, Gerard

    AU - Salvadó, M. Josepa

    AU - Arola, Lluis

    AU - Pinent, Monserrat

    AU - Ardévol, Anna

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    Montagut Pino G, Bladé C, Blay M, Fernández-Larrea J, Pujadas G, Salvadó MJ et al. Effects of a grapeseed procyanidin extract (GSPE) on insulin resistance. Journal of Nutritional Biochemistry. 2010 Oct;21(10):961-967. https://doi.org/10.1016/j.jnutbio.2009.08.001