Effects of culture conditions on the proliferation, morphology and migration of bovine aortic endothelial cells

A M Schor, S L Schor, T D Allen

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Various culture conditions, such as the presence of ascorbic acid, initial plating density and the nature of the substratum (plastic, gelatin or native collagen gels), influenced the growth, morphology and migration of three cloned populations of adult bovine aorta endothelial cells. Aorta endothelial cells showed two distinctive and reversible morphological phenotypes. Cells presenting a free apical surface were polygonal and formed sheets of overlapping or non-overlapping cells, depending on the culture conditions. When the cells were able to establish adhesive interactions over their entire cell surface they adopted an elongated shape and formed meshworks of interconnected 'sprouting' cells. The cells were capable of migrating into a collagen gel from both their basal and apical surfaces. Once in the gel, they formed characteristic, compact, three-dimensional meshworks.

Original languageEnglish
Pages (from-to)267-85
Number of pages19
JournalJournal of Cell Science
Volume62
Publication statusPublished - Jul 1983

Fingerprint

Endothelial Cells
Gels
Aorta
Collagen
Gelatin
Adhesives
Ascorbic Acid
Plastics
Phenotype
Growth
Population

Keywords

  • Animals
  • Aorta, Thoracic/cytology
  • Ascorbic Acid/pharmacology
  • Cattle
  • Cell Count
  • Cell Division
  • Cell Movement
  • Collagen
  • Culture Media
  • Endothelium/cytology
  • Microscopy, Electron, Scanning
  • Time Factors

Cite this

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abstract = "Various culture conditions, such as the presence of ascorbic acid, initial plating density and the nature of the substratum (plastic, gelatin or native collagen gels), influenced the growth, morphology and migration of three cloned populations of adult bovine aorta endothelial cells. Aorta endothelial cells showed two distinctive and reversible morphological phenotypes. Cells presenting a free apical surface were polygonal and formed sheets of overlapping or non-overlapping cells, depending on the culture conditions. When the cells were able to establish adhesive interactions over their entire cell surface they adopted an elongated shape and formed meshworks of interconnected 'sprouting' cells. The cells were capable of migrating into a collagen gel from both their basal and apical surfaces. Once in the gel, they formed characteristic, compact, three-dimensional meshworks.",
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Effects of culture conditions on the proliferation, morphology and migration of bovine aortic endothelial cells. / Schor, A M; Schor, S L; Allen, T D.

In: Journal of Cell Science, Vol. 62, 07.1983, p. 267-85.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of culture conditions on the proliferation, morphology and migration of bovine aortic endothelial cells

AU - Schor, A M

AU - Schor, S L

AU - Allen, T D

PY - 1983/7

Y1 - 1983/7

N2 - Various culture conditions, such as the presence of ascorbic acid, initial plating density and the nature of the substratum (plastic, gelatin or native collagen gels), influenced the growth, morphology and migration of three cloned populations of adult bovine aorta endothelial cells. Aorta endothelial cells showed two distinctive and reversible morphological phenotypes. Cells presenting a free apical surface were polygonal and formed sheets of overlapping or non-overlapping cells, depending on the culture conditions. When the cells were able to establish adhesive interactions over their entire cell surface they adopted an elongated shape and formed meshworks of interconnected 'sprouting' cells. The cells were capable of migrating into a collagen gel from both their basal and apical surfaces. Once in the gel, they formed characteristic, compact, three-dimensional meshworks.

AB - Various culture conditions, such as the presence of ascorbic acid, initial plating density and the nature of the substratum (plastic, gelatin or native collagen gels), influenced the growth, morphology and migration of three cloned populations of adult bovine aorta endothelial cells. Aorta endothelial cells showed two distinctive and reversible morphological phenotypes. Cells presenting a free apical surface were polygonal and formed sheets of overlapping or non-overlapping cells, depending on the culture conditions. When the cells were able to establish adhesive interactions over their entire cell surface they adopted an elongated shape and formed meshworks of interconnected 'sprouting' cells. The cells were capable of migrating into a collagen gel from both their basal and apical surfaces. Once in the gel, they formed characteristic, compact, three-dimensional meshworks.

KW - Animals

KW - Aorta, Thoracic/cytology

KW - Ascorbic Acid/pharmacology

KW - Cattle

KW - Cell Count

KW - Cell Division

KW - Cell Movement

KW - Collagen

KW - Culture Media

KW - Endothelium/cytology

KW - Microscopy, Electron, Scanning

KW - Time Factors

M3 - Article

C2 - 6619206

VL - 62

SP - 267

EP - 285

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

ER -