Abstract
Introduction: Regenerative endodontic procedures (REPs) aim to restore the structural and functionalintegrity of necrosed or infected dental pulp, while promoting root development and closure.Conventional treatment methods often fail to regenerate dental pulp tissues effectively. Thissystematic review investigates the efficacy of stem cell therapy in REPs.
Methodology: A literature search was conducted on six databases, including PubMed/MEDLINE, Scopus,PubMed Central, Google Scholar and ClinicalTrials.gov. The study was registered inPROSPERO and PRISMA guidelines were followed. 12 original studies comprising 1 clinicaltrial and 11 animal studies were included after applying inclusion and exclusion criteria. Thestudies evaluated different types of endodontic procedures, including pulp capping,pulpotomy, pulpectomy, and furcation repair, using various types of stem cells, such asdental pulp stem cells, bone marrow-derived stem cells and umbilical cord mesenchymalstem cells (UCMSCs). The outcomes assessed in the studies included pulpal regeneration,resolution of periapical radiolucency, and restoration of tooth vitality. The Systematic ReviewCenter for Laboratory and Animal Experimentation (SYRCLE) and Risk of Bias 2.0 was usedfor assessing the risk of bias in animal studies and randomised trials, respectively.
Results: The results showed that stem cell therapy has the potential to regenerate dental pulp tissuesand induce pulpal regeneration. However, the overall efficacy of stem cell therapy comparedto conventional methods was inconclusive. Some studies reported comparable outcomesbetween stem cell-based REPs and mineral trioxide aggregate, while others showedvariations in the formation of dentine bridges. The clinical trial using UCMSCs combined withplatelet-poor plasma showed positive outcomes in terms of dental pulp revascularisation andre-perfusion.
Conclusion: The long-term safety and clinical efficacy of stem cell therapy in REPs need furtherinvestigation before widespread clinical application.
Methodology: A literature search was conducted on six databases, including PubMed/MEDLINE, Scopus,PubMed Central, Google Scholar and ClinicalTrials.gov. The study was registered inPROSPERO and PRISMA guidelines were followed. 12 original studies comprising 1 clinicaltrial and 11 animal studies were included after applying inclusion and exclusion criteria. Thestudies evaluated different types of endodontic procedures, including pulp capping,pulpotomy, pulpectomy, and furcation repair, using various types of stem cells, such asdental pulp stem cells, bone marrow-derived stem cells and umbilical cord mesenchymalstem cells (UCMSCs). The outcomes assessed in the studies included pulpal regeneration,resolution of periapical radiolucency, and restoration of tooth vitality. The Systematic ReviewCenter for Laboratory and Animal Experimentation (SYRCLE) and Risk of Bias 2.0 was usedfor assessing the risk of bias in animal studies and randomised trials, respectively.
Results: The results showed that stem cell therapy has the potential to regenerate dental pulp tissuesand induce pulpal regeneration. However, the overall efficacy of stem cell therapy comparedto conventional methods was inconclusive. Some studies reported comparable outcomesbetween stem cell-based REPs and mineral trioxide aggregate, while others showedvariations in the formation of dentine bridges. The clinical trial using UCMSCs combined withplatelet-poor plasma showed positive outcomes in terms of dental pulp revascularisation andre-perfusion.
Conclusion: The long-term safety and clinical efficacy of stem cell therapy in REPs need furtherinvestigation before widespread clinical application.
| Original language | English |
|---|---|
| Article number | 102125 |
| Number of pages | 14 |
| Journal | Journal of Evidence-Based Dental Practice |
| Volume | 25 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 13 Mar 2025 |
Keywords
- Dental Pulp Stem Cells
- Bone-Marrow Stem Cells
- Umbilical Cord
- Stem Cells
- Pulp Regeneration
- Regenerative Endodonitcs
- Root Canal Therapy