TY - JOUR
T1 - Element localisation in polymorphic fungi using the oxford scanning proton microprobe
AU - Brunton, A.
AU - Watt, F.
AU - Grime, G. W.
AU - Gadd, G. M.
N1 - Funding Information:
G.M.G. acknowledges financial support from the University of Dundce Research Initiatives Fund and the Natural Environment Research Council (GR3/6060). A.H.B. gratefully acknowledges the receipt of a NERC postgraduate studentship.
Copyright © 1988 Published by Elsevier B.V.
PY - 1988/3/2
Y1 - 1988/3/2
N2 - Elemental concentrations and their distribution in different cell types of Ophiostoma ulmi were determined using the Oxford scanning proton microprobe. In all cell types i.e. germ-tubes and hyphae, K, P and S were major constituents and these, and other elements, were not distributed uniformly throughout the cells. In many cases, and especially for K and P, patterns of localisation could be correlated with vacuolation and/or a high cell solids content e.g. at germ-tube or hyphal tips. Other elements e.g. Cu and Fe appeared to be mainly located in the cytoplasm. Glutaraldehyde fixation appeared to induce some K loss from the cells although regions of K localisation were unaffected.
AB - Elemental concentrations and their distribution in different cell types of Ophiostoma ulmi were determined using the Oxford scanning proton microprobe. In all cell types i.e. germ-tubes and hyphae, K, P and S were major constituents and these, and other elements, were not distributed uniformly throughout the cells. In many cases, and especially for K and P, patterns of localisation could be correlated with vacuolation and/or a high cell solids content e.g. at germ-tube or hyphal tips. Other elements e.g. Cu and Fe appeared to be mainly located in the cytoplasm. Glutaraldehyde fixation appeared to induce some K loss from the cells although regions of K localisation were unaffected.
UR - http://www.scopus.com/inward/record.url?scp=0023310059&partnerID=8YFLogxK
U2 - 10.1016/0168-583X(88)90028-6
DO - 10.1016/0168-583X(88)90028-6
M3 - Article
AN - SCOPUS:0023310059
SN - 0168-583X
VL - 30
SP - 378
EP - 382
JO - Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
JF - Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
IS - 3
ER -