Elemental concentrations and their distribution in different cell types of Ophiostoma ulmi were determined using the Oxford scanning proton microprobe. In all cell types i.e. germ-tubes and hyphae, K, P and S were major constituents and these, and other elements, were not distributed uniformly throughout the cells. In many cases, and especially for K and P, patterns of localisation could be correlated with vacuolation and/or a high cell solids content e.g. at germ-tube or hyphal tips. Other elements e.g. Cu and Fe appeared to be mainly located in the cytoplasm. Glutaraldehyde fixation appeared to induce some K loss from the cells although regions of K localisation were unaffected.
|Number of pages||5|
|Journal||Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms|
|Publication status||Published - 2 Mar 1988|