Element localisation in polymorphic fungi using the oxford scanning proton microprobe

A. Brunton (Lead / Corresponding author), F. Watt, G. W. Grime, G. M. Gadd

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    3 Citations (Scopus)

    Abstract

    Elemental concentrations and their distribution in different cell types of Ophiostoma ulmi were determined using the Oxford scanning proton microprobe. In all cell types i.e. germ-tubes and hyphae, K, P and S were major constituents and these, and other elements, were not distributed uniformly throughout the cells. In many cases, and especially for K and P, patterns of localisation could be correlated with vacuolation and/or a high cell solids content e.g. at germ-tube or hyphal tips. Other elements e.g. Cu and Fe appeared to be mainly located in the cytoplasm. Glutaraldehyde fixation appeared to induce some K loss from the cells although regions of K localisation were unaffected.

    Original languageEnglish
    Pages (from-to)378-382
    Number of pages5
    JournalNuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
    Volume30
    Issue number3
    DOIs
    Publication statusPublished - 2 Mar 1988

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