Element localisation in polymorphic fungi using the oxford scanning proton microprobe

A. Brunton; F. Watt; G. W. Grime; G. M. Gadd

doi:10.1016/0168-583X(88)90028-6

Element localisation in polymorphic fungi using the oxford scanning proton microprobe

A. Brunton (Lead / Corresponding author)
, F. Watt
, G. W. Grime
, G. M. Gadd

University of Dundee

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Elemental concentrations and their distribution in different cell types of Ophiostoma ulmi were determined using the Oxford scanning proton microprobe. In all cell types i.e. germ-tubes and hyphae, K, P and S were major constituents and these, and other elements, were not distributed uniformly throughout the cells. In many cases, and especially for K and P, patterns of localisation could be correlated with vacuolation and/or a high cell solids content e.g. at germ-tube or hyphal tips. Other elements e.g. Cu and Fe appeared to be mainly located in the cytoplasm. Glutaraldehyde fixation appeared to induce some K loss from the cells although regions of K localisation were unaffected.

Original language	English
Pages (from-to)	378-382
Number of pages	5
Journal	Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
Volume	30
Issue number	3
DOIs	https://doi.org/10.1016/0168-583X(88)90028-6
Publication status	Published - 2 Mar 1988

ASJC Scopus subject areas

Nuclear and High Energy Physics
Instrumentation

Access to Document

10.1016/0168-583X(88)90028-6Licence: Elsevier User Licence

Cite this

@article{164fbede7411405f859544b3099fcc53,

title = "Element localisation in polymorphic fungi using the oxford scanning proton microprobe",

abstract = "Elemental concentrations and their distribution in different cell types of Ophiostoma ulmi were determined using the Oxford scanning proton microprobe. In all cell types i.e. germ-tubes and hyphae, K, P and S were major constituents and these, and other elements, were not distributed uniformly throughout the cells. In many cases, and especially for K and P, patterns of localisation could be correlated with vacuolation and/or a high cell solids content e.g. at germ-tube or hyphal tips. Other elements e.g. Cu and Fe appeared to be mainly located in the cytoplasm. Glutaraldehyde fixation appeared to induce some K loss from the cells although regions of K localisation were unaffected.",

author = "A. Brunton and F. Watt and Grime, \{G. W.\} and Gadd, \{G. M.\}",

note = "Funding Information: G.M.G. acknowledges financial support from the University of Dundce Research Initiatives Fund and the Natural Environment Research Council (GR3/6060). A.H.B. gratefully acknowledges the receipt of a NERC postgraduate studentship. Copyright {\textcopyright} 1988 Published by Elsevier B.V.",

year = "1988",

month = mar,

day = "2",

doi = "10.1016/0168-583X(88)90028-6",

language = "English",

volume = "30",

pages = "378--382",

number = "3",

}

Element localisation in polymorphic fungi using the oxford scanning proton microprobe. / Brunton, A. (Lead / Corresponding author); Watt, F.; Grime, G. W. et al.
In: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, Vol. 30, No. 3, 02.03.1988, p. 378-382.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Element localisation in polymorphic fungi using the oxford scanning proton microprobe

AU - Brunton, A.

AU - Watt, F.

AU - Grime, G. W.

AU - Gadd, G. M.

N1 - Funding Information: G.M.G. acknowledges financial support from the University of Dundce Research Initiatives Fund and the Natural Environment Research Council (GR3/6060). A.H.B. gratefully acknowledges the receipt of a NERC postgraduate studentship. Copyright © 1988 Published by Elsevier B.V.

PY - 1988/3/2

Y1 - 1988/3/2

N2 - Elemental concentrations and their distribution in different cell types of Ophiostoma ulmi were determined using the Oxford scanning proton microprobe. In all cell types i.e. germ-tubes and hyphae, K, P and S were major constituents and these, and other elements, were not distributed uniformly throughout the cells. In many cases, and especially for K and P, patterns of localisation could be correlated with vacuolation and/or a high cell solids content e.g. at germ-tube or hyphal tips. Other elements e.g. Cu and Fe appeared to be mainly located in the cytoplasm. Glutaraldehyde fixation appeared to induce some K loss from the cells although regions of K localisation were unaffected.

AB - Elemental concentrations and their distribution in different cell types of Ophiostoma ulmi were determined using the Oxford scanning proton microprobe. In all cell types i.e. germ-tubes and hyphae, K, P and S were major constituents and these, and other elements, were not distributed uniformly throughout the cells. In many cases, and especially for K and P, patterns of localisation could be correlated with vacuolation and/or a high cell solids content e.g. at germ-tube or hyphal tips. Other elements e.g. Cu and Fe appeared to be mainly located in the cytoplasm. Glutaraldehyde fixation appeared to induce some K loss from the cells although regions of K localisation were unaffected.

UR - https://www.scopus.com/pages/publications/0023310059

U2 - 10.1016/0168-583X(88)90028-6

DO - 10.1016/0168-583X(88)90028-6

M3 - Article

AN - SCOPUS:0023310059

SN - 0168-583X

VL - 30

SP - 378

EP - 382

JO - Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms

JF - Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms

IS - 3

ER -

Element localisation in polymorphic fungi using the oxford scanning proton microprobe

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this