Elevated expression of the genes encoding TNF-alpha and thromboxane synthase in leucocytes from patients with systemic sclerosis

V. Young; M. Ho; H. Vosper; J. J. F. Belch; C. N. A. Palmer

doi:10.1093/rheumatology/41.8.869

Elevated expression of the genes encoding TNF-alpha and thromboxane synthase in leucocytes from patients with systemic sclerosis

V. Young, M. Ho, H. Vosper, J. J. F. Belch, C. N. A. Palmer

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Objective. To determine the expression of molecular markers of prostanoid/fatty acid signalling in leucocytes of patients with systemic sclerosis (SSc).

Methods. Gene expression in patient leucocytes was analysed using real-time fluorescence reverse transcriptase polymerase chain reaction for tumour necrosis factor alpha (TNF-alpha), thromboxane synthase (TXAS, CYP5A), prostacyclin synthase (CYP8A), monocyte chemoattractant protein-1 (MCP-1), peroxisome proliferator-activated receptors (PPAR) alpha, delta and ?, low-density lipoprotein-associated lipoprotein lipase A₂ (LDL-PLA₂), apolipoprotein E (apoE) and cholesterol 27-hydroxylase (CYP27).

Results. Both TNF-alpha and TXAS showed an increase in mean expression in the diseased group (6.3-fold and 5.6-fold respectively, P < 0.0001). These two markers, along with CYP27, PPAR? and apoE, provided predictive markers for the development of carotid artery disease within the SSc patient population.

Conclusion. The elevated levels of TNF-alpha and thromboxane seen in SSc patient sera are paralleled by increases in the expression of the appropriate genes in leucocytes. This method will allow us to screen for a large number of candidate markers of disease in order to increase our understanding of the processes underlying the pathology of SSc.

Original language	English
Pages (from-to)	869-875
Number of pages	7
Journal	Rheumatology
Volume	41
Issue number	8
DOIs	https://doi.org/10.1093/rheumatology/41.8.869
Publication status	Published - Aug 2002

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@article{6bab9a6c2f104828bbd1e10e2c7dee4b,

title = "Elevated expression of the genes encoding TNF-alpha and thromboxane synthase in leucocytes from patients with systemic sclerosis",

abstract = "Objective. To determine the expression of molecular markers of prostanoid/fatty acid signalling in leucocytes of patients with systemic sclerosis (SSc). Methods. Gene expression in patient leucocytes was analysed using real-time fluorescence reverse transcriptase polymerase chain reaction for tumour necrosis factor alpha (TNF-alpha), thromboxane synthase (TXAS, CYP5A), prostacyclin synthase (CYP8A), monocyte chemoattractant protein-1 (MCP-1), peroxisome proliferator-activated receptors (PPAR) alpha, delta and ?, low-density lipoprotein-associated lipoprotein lipase A2 (LDL-PLA2), apolipoprotein E (apoE) and cholesterol 27-hydroxylase (CYP27). Results. Both TNF-alpha and TXAS showed an increase in mean expression in the diseased group (6.3-fold and 5.6-fold respectively, P < 0.0001). These two markers, along with CYP27, PPAR? and apoE, provided predictive markers for the development of carotid artery disease within the SSc patient population. Conclusion. The elevated levels of TNF-alpha and thromboxane seen in SSc patient sera are paralleled by increases in the expression of the appropriate genes in leucocytes. This method will allow us to screen for a large number of candidate markers of disease in order to increase our understanding of the processes underlying the pathology of SSc.",

author = "V. Young and M. Ho and H. Vosper and Belch, {J. J. F.} and Palmer, {C. N. A.}",

year = "2002",

month = aug,

doi = "10.1093/rheumatology/41.8.869",

language = "English",

volume = "41",

pages = "869--875",

journal = "Rheumatology",

issn = "1462-0324",

publisher = "Oxford University Press",

number = "8",

}

TY - JOUR

T1 - Elevated expression of the genes encoding TNF-alpha and thromboxane synthase in leucocytes from patients with systemic sclerosis

AU - Young, V.

AU - Ho, M.

AU - Vosper, H.

AU - Belch, J. J. F.

AU - Palmer, C. N. A.

PY - 2002/8

Y1 - 2002/8

N2 - Objective. To determine the expression of molecular markers of prostanoid/fatty acid signalling in leucocytes of patients with systemic sclerosis (SSc). Methods. Gene expression in patient leucocytes was analysed using real-time fluorescence reverse transcriptase polymerase chain reaction for tumour necrosis factor alpha (TNF-alpha), thromboxane synthase (TXAS, CYP5A), prostacyclin synthase (CYP8A), monocyte chemoattractant protein-1 (MCP-1), peroxisome proliferator-activated receptors (PPAR) alpha, delta and ?, low-density lipoprotein-associated lipoprotein lipase A2 (LDL-PLA2), apolipoprotein E (apoE) and cholesterol 27-hydroxylase (CYP27). Results. Both TNF-alpha and TXAS showed an increase in mean expression in the diseased group (6.3-fold and 5.6-fold respectively, P < 0.0001). These two markers, along with CYP27, PPAR? and apoE, provided predictive markers for the development of carotid artery disease within the SSc patient population. Conclusion. The elevated levels of TNF-alpha and thromboxane seen in SSc patient sera are paralleled by increases in the expression of the appropriate genes in leucocytes. This method will allow us to screen for a large number of candidate markers of disease in order to increase our understanding of the processes underlying the pathology of SSc.

AB - Objective. To determine the expression of molecular markers of prostanoid/fatty acid signalling in leucocytes of patients with systemic sclerosis (SSc). Methods. Gene expression in patient leucocytes was analysed using real-time fluorescence reverse transcriptase polymerase chain reaction for tumour necrosis factor alpha (TNF-alpha), thromboxane synthase (TXAS, CYP5A), prostacyclin synthase (CYP8A), monocyte chemoattractant protein-1 (MCP-1), peroxisome proliferator-activated receptors (PPAR) alpha, delta and ?, low-density lipoprotein-associated lipoprotein lipase A2 (LDL-PLA2), apolipoprotein E (apoE) and cholesterol 27-hydroxylase (CYP27). Results. Both TNF-alpha and TXAS showed an increase in mean expression in the diseased group (6.3-fold and 5.6-fold respectively, P < 0.0001). These two markers, along with CYP27, PPAR? and apoE, provided predictive markers for the development of carotid artery disease within the SSc patient population. Conclusion. The elevated levels of TNF-alpha and thromboxane seen in SSc patient sera are paralleled by increases in the expression of the appropriate genes in leucocytes. This method will allow us to screen for a large number of candidate markers of disease in order to increase our understanding of the processes underlying the pathology of SSc.

U2 - 10.1093/rheumatology/41.8.869

DO - 10.1093/rheumatology/41.8.869

M3 - Article

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VL - 41

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JO - Rheumatology

JF - Rheumatology

SN - 1462-0324

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