Abstract
Objective. To determine the expression of molecular markers of prostanoid/fatty acid signalling in leucocytes of patients with systemic sclerosis (SSc).
Methods. Gene expression in patient leucocytes was analysed using real-time fluorescence reverse transcriptase polymerase chain reaction for tumour necrosis factor alpha (TNF-alpha), thromboxane synthase (TXAS, CYP5A), prostacyclin synthase (CYP8A), monocyte chemoattractant protein-1 (MCP-1), peroxisome proliferator-activated receptors (PPAR) alpha, delta and ?, low-density lipoprotein-associated lipoprotein lipase A2 (LDL-PLA2), apolipoprotein E (apoE) and cholesterol 27-hydroxylase (CYP27).
Results. Both TNF-alpha and TXAS showed an increase in mean expression in the diseased group (6.3-fold and 5.6-fold respectively, P < 0.0001). These two markers, along with CYP27, PPAR? and apoE, provided predictive markers for the development of carotid artery disease within the SSc patient population.
Conclusion. The elevated levels of TNF-alpha and thromboxane seen in SSc patient sera are paralleled by increases in the expression of the appropriate genes in leucocytes. This method will allow us to screen for a large number of candidate markers of disease in order to increase our understanding of the processes underlying the pathology of SSc.
| Original language | English |
|---|---|
| Pages (from-to) | 869-875 |
| Number of pages | 7 |
| Journal | Rheumatology |
| Volume | 41 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 2002 |
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Elevated expression of the genes encoding TNF-alpha and thromboxane synthase in leucocytes from patients with systemic sclerosis. / Young, V.; Ho, M.; Vosper, H.; Belch, J. J. F.; Palmer, C. N. A.
In: Rheumatology, Vol. 41, No. 8, 08.2002, p. 869-875.Research output: Contribution to journal › Article
TY - JOUR
T1 - Elevated expression of the genes encoding TNF-alpha and thromboxane synthase in leucocytes from patients with systemic sclerosis
AU - Young, V.
AU - Ho, M.
AU - Vosper, H.
AU - Belch, J. J. F.
AU - Palmer, C. N. A.
PY - 2002/8
Y1 - 2002/8
N2 - Objective. To determine the expression of molecular markers of prostanoid/fatty acid signalling in leucocytes of patients with systemic sclerosis (SSc). Methods. Gene expression in patient leucocytes was analysed using real-time fluorescence reverse transcriptase polymerase chain reaction for tumour necrosis factor alpha (TNF-alpha), thromboxane synthase (TXAS, CYP5A), prostacyclin synthase (CYP8A), monocyte chemoattractant protein-1 (MCP-1), peroxisome proliferator-activated receptors (PPAR) alpha, delta and ?, low-density lipoprotein-associated lipoprotein lipase A2 (LDL-PLA2), apolipoprotein E (apoE) and cholesterol 27-hydroxylase (CYP27). Results. Both TNF-alpha and TXAS showed an increase in mean expression in the diseased group (6.3-fold and 5.6-fold respectively, P < 0.0001). These two markers, along with CYP27, PPAR? and apoE, provided predictive markers for the development of carotid artery disease within the SSc patient population. Conclusion. The elevated levels of TNF-alpha and thromboxane seen in SSc patient sera are paralleled by increases in the expression of the appropriate genes in leucocytes. This method will allow us to screen for a large number of candidate markers of disease in order to increase our understanding of the processes underlying the pathology of SSc.
AB - Objective. To determine the expression of molecular markers of prostanoid/fatty acid signalling in leucocytes of patients with systemic sclerosis (SSc). Methods. Gene expression in patient leucocytes was analysed using real-time fluorescence reverse transcriptase polymerase chain reaction for tumour necrosis factor alpha (TNF-alpha), thromboxane synthase (TXAS, CYP5A), prostacyclin synthase (CYP8A), monocyte chemoattractant protein-1 (MCP-1), peroxisome proliferator-activated receptors (PPAR) alpha, delta and ?, low-density lipoprotein-associated lipoprotein lipase A2 (LDL-PLA2), apolipoprotein E (apoE) and cholesterol 27-hydroxylase (CYP27). Results. Both TNF-alpha and TXAS showed an increase in mean expression in the diseased group (6.3-fold and 5.6-fold respectively, P < 0.0001). These two markers, along with CYP27, PPAR? and apoE, provided predictive markers for the development of carotid artery disease within the SSc patient population. Conclusion. The elevated levels of TNF-alpha and thromboxane seen in SSc patient sera are paralleled by increases in the expression of the appropriate genes in leucocytes. This method will allow us to screen for a large number of candidate markers of disease in order to increase our understanding of the processes underlying the pathology of SSc.
U2 - 10.1093/rheumatology/41.8.869
DO - 10.1093/rheumatology/41.8.869
M3 - Article
C2 - 12154203
VL - 41
SP - 869
EP - 875
JO - Rheumatology
JF - Rheumatology
SN - 1462-0324
IS - 8
ER -