Elevated levels of tryparedoxin peroxidase in antimony unresponsive Leishmania donovani field isolates

Susan Wyllie, Goutam Mandal, Neeloo Singh, Shyam Sundar, Alan H. Fairlamb, Mitali Chatterjee

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    52 Citations (Scopus)

    Abstract

    Enhancement of the anti-oxidant metabolism of Leishmania parasites, dependent upon the unique dithiol trypanothione, has been implicated in laboratory-generated antimony resistance. Here, the role of the trypanothione-dependent anti-oxidant pathway is studied in antimony-resistant clinical isolates. Elevated levels of tryparedoxin and tryparedoxin peroxidase, key enzymes in hydroperoxide detoxification, were observed in antimonial resistant parasites resulting in an increased metabolism of peroxides. These data suggest that enhanced anti-oxidant defences may play a significant role in clinical resistance to antimonials. (C) 2010 Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)162-164
    Number of pages3
    JournalMolecular and Biochemical Parasitology
    Volume173
    Issue number2
    DOIs
    Publication statusPublished - Oct 2010

    Keywords

    • Antimonial resistance
    • Leishmania donovani
    • Tryparedoxin peroxidase
    • ARSENITE-RESISTANT LEISHMANIA
    • GAMMA-GLUTAMYLCYSTEINE SYNTHETASE
    • DRUG-RESISTANCE
    • ORNITHINE-DECARBOXYLASE
    • PARASITIC PROTOZOA
    • TRYPANOTHIONE
    • METABOLISM
    • GENE
    • OVEREXPRESSION
    • EXPRESSION

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