In Vitro and In Vivo studies of trypanocidal activity of dietary isothiocyanates

Dietmar Steverding, Sarah Michaels, Kevin D. Read

    Research output: Contribution to journalLetter

    2 Citations (Scopus)

    Abstract

    Six dietary isothiocyanates, allyl-isothiocyanate, benzyl-isothiocyanate, phenylethyl-isothiocyanate, sulforaphane, erucin, and iberin, were tested for their trypanocidal activities in vitro using culture-adapted bloodstream forms of Trypanosoma brucei. All isothiocyanates showed a dose-dependent effect on the growth of trypanosomes. Five compounds displayed MIC values of 10?µM and GI50 values of around 1.5µM, while allyl-isothiocyanate exhibited values of 100 and 11µM, respectively. The compounds showed similar cytotoxic activities against human HL-60 cells with GI50 values of 1-4µM and MIC values of 10-100µM. Short-term experiments revealed that, with the exception of allyl-isothiocyanate, isothiocyanates at a concentration of 10µM kill trypanosomes within 1-4h of incubation. In contrast, HL-60 cells were not affected by any of the compounds in short-term incubation experiments. Sulforaphane, the most intensively studied isothiocyanate, was also investigated for its in vivo trypanocidal activity. However, administration of 50mg/kg sulforaphane orally or intraperitoneally for four days had no effect on the parasitaemia in mice infected with T. brucei compared to control animals treated with vehicle alone.

    Original languageEnglish
    JournalPlanta Medica
    DOIs
    Publication statusPublished - 22 Jan 2014

    Fingerprint Dive into the research topics of '<em>In Vitro </em>and <em>In Vivo</em> studies of trypanocidal activity of dietary isothiocyanates'. Together they form a unique fingerprint.

  • Profiles

    No photo of Kevin Read

    Read, Kevin

    Person: Academic

    Cite this