In Vitro and In Vivo studies of trypanocidal activity of dietary isothiocyanates

Dietmar Steverding, Sarah Michaels, Kevin D. Read

    Research output: Contribution to journalLetter

    2 Citations (Scopus)

    Abstract

    Six dietary isothiocyanates, allyl-isothiocyanate, benzyl-isothiocyanate, phenylethyl-isothiocyanate, sulforaphane, erucin, and iberin, were tested for their trypanocidal activities in vitro using culture-adapted bloodstream forms of Trypanosoma brucei. All isothiocyanates showed a dose-dependent effect on the growth of trypanosomes. Five compounds displayed MIC values of 10?µM and GI50 values of around 1.5µM, while allyl-isothiocyanate exhibited values of 100 and 11µM, respectively. The compounds showed similar cytotoxic activities against human HL-60 cells with GI50 values of 1-4µM and MIC values of 10-100µM. Short-term experiments revealed that, with the exception of allyl-isothiocyanate, isothiocyanates at a concentration of 10µM kill trypanosomes within 1-4h of incubation. In contrast, HL-60 cells were not affected by any of the compounds in short-term incubation experiments. Sulforaphane, the most intensively studied isothiocyanate, was also investigated for its in vivo trypanocidal activity. However, administration of 50mg/kg sulforaphane orally or intraperitoneally for four days had no effect on the parasitaemia in mice infected with T. brucei compared to control animals treated with vehicle alone.

    Original languageEnglish
    JournalPlanta Medica
    DOIs
    Publication statusPublished - 22 Jan 2014

    Fingerprint

    Isothiocyanates
    Trypanosoma brucei brucei
    Trypanosomiasis
    HL-60 Cells
    Parasitemia
    Human Activities
    Animals
    Experiments
    Growth
    sulforafan
    In Vitro Techniques
    allyl isothiocyanate

    Cite this

    @article{77294a73cfbe45efab117fc28a222cd7,
    title = "In Vitro and In Vivo studies of trypanocidal activity of dietary isothiocyanates",
    abstract = "Six dietary isothiocyanates, allyl-isothiocyanate, benzyl-isothiocyanate, phenylethyl-isothiocyanate, sulforaphane, erucin, and iberin, were tested for their trypanocidal activities in vitro using culture-adapted bloodstream forms of Trypanosoma brucei. All isothiocyanates showed a dose-dependent effect on the growth of trypanosomes. Five compounds displayed MIC values of 10?µM and GI50 values of around 1.5µM, while allyl-isothiocyanate exhibited values of 100 and 11µM, respectively. The compounds showed similar cytotoxic activities against human HL-60 cells with GI50 values of 1-4µM and MIC values of 10-100µM. Short-term experiments revealed that, with the exception of allyl-isothiocyanate, isothiocyanates at a concentration of 10µM kill trypanosomes within 1-4h of incubation. In contrast, HL-60 cells were not affected by any of the compounds in short-term incubation experiments. Sulforaphane, the most intensively studied isothiocyanate, was also investigated for its in vivo trypanocidal activity. However, administration of 50mg/kg sulforaphane orally or intraperitoneally for four days had no effect on the parasitaemia in mice infected with T. brucei compared to control animals treated with vehicle alone.",
    author = "Dietmar Steverding and Sarah Michaels and Read, {Kevin D.}",
    year = "2014",
    month = "1",
    day = "22",
    doi = "10.1055/s-0033-1360262",
    language = "English",
    journal = "Planta Medica",
    issn = "0032-0943",
    publisher = "Thieme Medical Publishers",

    }

    In Vitro and In Vivo studies of trypanocidal activity of dietary isothiocyanates. / Steverding, Dietmar; Michaels, Sarah; Read, Kevin D.

    In: Planta Medica, 22.01.2014.

    Research output: Contribution to journalLetter

    TY - JOUR

    T1 - In Vitro and In Vivo studies of trypanocidal activity of dietary isothiocyanates

    AU - Steverding, Dietmar

    AU - Michaels, Sarah

    AU - Read, Kevin D.

    PY - 2014/1/22

    Y1 - 2014/1/22

    N2 - Six dietary isothiocyanates, allyl-isothiocyanate, benzyl-isothiocyanate, phenylethyl-isothiocyanate, sulforaphane, erucin, and iberin, were tested for their trypanocidal activities in vitro using culture-adapted bloodstream forms of Trypanosoma brucei. All isothiocyanates showed a dose-dependent effect on the growth of trypanosomes. Five compounds displayed MIC values of 10?µM and GI50 values of around 1.5µM, while allyl-isothiocyanate exhibited values of 100 and 11µM, respectively. The compounds showed similar cytotoxic activities against human HL-60 cells with GI50 values of 1-4µM and MIC values of 10-100µM. Short-term experiments revealed that, with the exception of allyl-isothiocyanate, isothiocyanates at a concentration of 10µM kill trypanosomes within 1-4h of incubation. In contrast, HL-60 cells were not affected by any of the compounds in short-term incubation experiments. Sulforaphane, the most intensively studied isothiocyanate, was also investigated for its in vivo trypanocidal activity. However, administration of 50mg/kg sulforaphane orally or intraperitoneally for four days had no effect on the parasitaemia in mice infected with T. brucei compared to control animals treated with vehicle alone.

    AB - Six dietary isothiocyanates, allyl-isothiocyanate, benzyl-isothiocyanate, phenylethyl-isothiocyanate, sulforaphane, erucin, and iberin, were tested for their trypanocidal activities in vitro using culture-adapted bloodstream forms of Trypanosoma brucei. All isothiocyanates showed a dose-dependent effect on the growth of trypanosomes. Five compounds displayed MIC values of 10?µM and GI50 values of around 1.5µM, while allyl-isothiocyanate exhibited values of 100 and 11µM, respectively. The compounds showed similar cytotoxic activities against human HL-60 cells with GI50 values of 1-4µM and MIC values of 10-100µM. Short-term experiments revealed that, with the exception of allyl-isothiocyanate, isothiocyanates at a concentration of 10µM kill trypanosomes within 1-4h of incubation. In contrast, HL-60 cells were not affected by any of the compounds in short-term incubation experiments. Sulforaphane, the most intensively studied isothiocyanate, was also investigated for its in vivo trypanocidal activity. However, administration of 50mg/kg sulforaphane orally or intraperitoneally for four days had no effect on the parasitaemia in mice infected with T. brucei compared to control animals treated with vehicle alone.

    U2 - 10.1055/s-0033-1360262

    DO - 10.1055/s-0033-1360262

    M3 - Letter

    JO - Planta Medica

    JF - Planta Medica

    SN - 0032-0943

    ER -