Enabling technologies for manipulating multiple genes on complex pathways

Claire Halpin (Lead / Corresponding author), Abdellah Barakate, Barak M. Askari, James C. Abbott, Martin D. Ryan

Research output: Contribution to journalArticlepeer-review

73 Citations (Scopus)

Abstract

Many complex biochemical pathways in plants have now been manipulated genetically, usually by suppression or over-expression of single genes. Further exploitation of the potential for plant genetic manipulation, both as a research tool and as a vehicle for plant biotechnology, will require the co-ordinate manipulation of multiple genes on a pathway. This goal is currently very difficult to achieve. A number of approaches have been taken to combine or 'pyramid' transgenes in one plant and have met with varying degrees of success. These approaches include sexual crossing, re-transformation, co-transformation and the Use of linked transgenes. Novel, alternative 'enabling' technologies are also being developed that aim to use single transgenes to manipulate the expression of multiple genes. A chimeric transgene with linked partial gene sequences placed under the control of a single promoter can be used to co-ordinately suppress numerous plant endogenous genes. Constructs modelled on viral polyproteins can be used to simultaneously introduce multiple protein-coding genes into plant cells. In the course of our work on the lignin biosynthetic pathway, we have tested both conventional and novel methods for achieving co-ordinate suppression or over-expression of up to three plant lignin genes. In this article we review the literature concerning the manipulation of multiple genes in plants. We also report on our own experiences and results using different methods to perform directed manipulation of lignin biosyhthesis in tobacco.

Original languageEnglish
Pages (from-to)295-310
Number of pages16
JournalPlant Molecular Biology
Volume47
Issue number1-2
DOIs
Publication statusPublished - Sep 2001

Keywords

  • Co-ordinate expression
  • Lignin
  • Multiple transgenes
  • Polyproteins
  • Pyramiding
  • Transgene silencing

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