TY - JOUR
T1 - Endemic Mimosa species from Mexico prefer alphaproteobacterial rhizobial symbionts
AU - Bontemps, Cyril
AU - Rogel, Marco Antonio
AU - Wiechmann, Anja
AU - Mussabekova, Assel
AU - Moody, Sarah
AU - Simon, Marcelo F.
AU - Moulin, Lionel
AU - Elliott, Geoffrey N.
AU - Lacercat-Didier, Laurence
AU - Dasilva, Cindy
AU - Grether, Rosaura
AU - Camargo-Ricalde, Sara L.
AU - Chen, Weimin
AU - Sprent, Janet I.
AU - Martínez-Romero, Esperanza
AU - Young, J. Peter W.
AU - James, Euan K.
PY - 2016/1
Y1 - 2016/1
N2 - The legume genus Mimosa has > 500 species, with two major centres of diversity, Brazil (c. 350 spp.) and Mexico (c. 100 spp.). In Brazil most species are nodulated by Burkholderia. Here we asked whether this is also true of native and endemic Mexican species. We have tested this apparent affinity for betaproteobacteria by examining the symbionts of native and endemic species of Mimosa in Mexico, especially from the central highlands where Mimosa spp. have diversified. Nodules were tested for betaproteobacteria using in situ immunolocalization. Rhizobia isolated from the nodules were genetically characterized and tested for their ability to nodulate Mimosa spp. Immunological analysis of 25 host taxa suggested that most (including all the highland endemics) were not nodulated by betaproteobacteria. Phylogenetic analyses of 16S rRNA, recA, nodA, nodC and nifH genes from 87 strains isolated from 20 taxa confirmed that the endemic Mexican Mimosa species favoured alphaproteobacteria in the genera Rhizobium and Ensifer: this was confirmed by nodulation tests. Host phylogeny, geographic isolation and coevolution with symbionts derived from very different soils have potentially contributed to the striking difference in the choice of symbiotic partners by Mexican and Brazilian Mimosa species.
AB - The legume genus Mimosa has > 500 species, with two major centres of diversity, Brazil (c. 350 spp.) and Mexico (c. 100 spp.). In Brazil most species are nodulated by Burkholderia. Here we asked whether this is also true of native and endemic Mexican species. We have tested this apparent affinity for betaproteobacteria by examining the symbionts of native and endemic species of Mimosa in Mexico, especially from the central highlands where Mimosa spp. have diversified. Nodules were tested for betaproteobacteria using in situ immunolocalization. Rhizobia isolated from the nodules were genetically characterized and tested for their ability to nodulate Mimosa spp. Immunological analysis of 25 host taxa suggested that most (including all the highland endemics) were not nodulated by betaproteobacteria. Phylogenetic analyses of 16S rRNA, recA, nodA, nodC and nifH genes from 87 strains isolated from 20 taxa confirmed that the endemic Mexican Mimosa species favoured alphaproteobacteria in the genera Rhizobium and Ensifer: this was confirmed by nodulation tests. Host phylogeny, geographic isolation and coevolution with symbionts derived from very different soils have potentially contributed to the striking difference in the choice of symbiotic partners by Mexican and Brazilian Mimosa species.
KW - Ensifer
KW - Mimosa
KW - Rhizobium
KW - Burkholderia
KW - Legume nodulation
KW - Nitrogen (N) fixation
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-84949319544&origin=resultslist&sort=plf-f&src=s&st1=Endemic+Mimosa+species+from+Mexico+prefer+alphaproteobacterial+rhizobial+symbionts&st2=&sid=0C460C4464C3A0AE1B51E66A158B5715.wsnAw8kcdt7IPYLO0V48gA%3a20&sot=b&sdt=b&sl=97&s=TITLE-ABS-KEY%28Endemic+Mimosa+species+from+Mexico+prefer+alphaproteobacterial+rhizobial+symbionts%29&relpos=0&citeCnt=5&searchTerm=
U2 - 10.1111/nph.13573
DO - 10.1111/nph.13573
M3 - Article
C2 - 26214613
VL - 209
SP - 319
EP - 333
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 1
ER -