Engineering heat tolerance in potato by temperature-dependent expression of a specific allele of HEAT SHOCK COGNATE 70

Almudena Trapero-Mozos, Wayne L. Morris, Laurence J. M. Ducreux, Karen McLean, Jennifer Stephens, Lesley Torrance, Glenn J. Bryan, Robert D. Hancock, Mark A. Taylor (Lead / Corresponding author)

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18 Citations (Scopus)
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Abstract

For many commercial potato cultivars, tuber yield is optimal at average day time temperatures in the range of 14-22⁰C. Further rises in ambient temperature can reduce or completely inhibit potato tuber production, with damaging consequences for both producer and consumer. The aim of this study was to use a genetic screen based on a model tuberisation assay to identify Quantitative Trait Loci (QTL) associated with enhanced tuber yield. A candidate gene encoding HSc70 was identified within one of the three QTL intervals associated with elevated yield in a Phureja-Tuberosum hybrid diploid potato population (06H1). A particular HSc70 allelic variant was linked to elevated yield in the 06H1 progeny. Expression of this allelic variant was much higher than other alleles, particularly on exposure to moderately elevated temperature. Transient expression of this allele in Nicotiana benthamiana resulted in significantly enhanced tolerance to elevated temperature. An TA repeat element was present in the promoter of this allele, but not in other HSc70 alleles identified in the population. Expression of the HSc70 allelic variant under its native promoter in the potato cultivar Desiree resulted in enhanced HSc70 expression at elevated temperature. This was reflected in greater tolerance to heat stress as determined by improved yield under moderately elevated temperature in a model nodal cutting tuberisation system and in plants grown from stem cuttings. Our results identify HSc70 expression level as a significant factor influencing yield stability under moderately elevated temperature and identify specific allelic variants of HSc70 for the induction of thermotolerance via conventional introgression or molecular breeding approaches. This article is protected by copyright. All rights reserved.

Original languageEnglish
Pages (from-to)197-207
Number of pages11
JournalPlant Biotechnology Journal
Volume16
Early online date16 May 2017
DOIs
Publication statusPublished - 20 Jun 2017

Keywords

  • Journal article 70
  • HEAT SHOCK COGNATE 70
  • Heat tolerance
  • Potato
  • Promoter
  • Quantitative trait locus
  • Yield

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    Trapero-Mozos, A., Morris, W. L., Ducreux, L. J. M., McLean, K., Stephens, J., Torrance, L., Bryan, G. J., Hancock, R. D., & Taylor, M. A. (2017). Engineering heat tolerance in potato by temperature-dependent expression of a specific allele of HEAT SHOCK COGNATE 70. Plant Biotechnology Journal, 16, 197-207. https://doi.org/10.1111/pbi.12760