Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]

Xanthi Tsampoula; Michael Mazilu; Tom Vettenburg; Frank Gunn-Moore; Kishan Dholakia

doi:10.1364/PRJ.1.000042

Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]

Xanthi Tsampoula, Michael Mazilu (Lead / Corresponding author), Tom Vettenburg, Frank Gunn-Moore, Kishan Dholakia

Physics

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.

Original language	English
Pages (from-to)	42-46
Number of pages	5
Journal	Photonics Research
Volume	1
Issue number	1
DOIs	https://doi.org/10.1364/PRJ.1.000042
Publication status	Published - 1 Jan 2013

Keywords

Diffractive optics
Laser beam shaping

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics

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10.1364/PRJ.1.000042

Cite this

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abstract = "We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.",

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AU - Dholakia, Kishan

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AB - We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.

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KW - Laser beam shaping

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Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]

Abstract

Keywords

ASJC Scopus subject areas

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