We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.
- Diffractive optics
- Laser beam shaping
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics