Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]

Xanthi Tsampoula; Michael Mazilu; Tom Vettenburg; Frank Gunn-Moore; Kishan Dholakia

doi:10.1364/PRJ.1.000042

Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]

Xanthi Tsampoula
, Michael Mazilu (Lead / Corresponding author)
, Tom Vettenburg
, Frank Gunn-Moore
, Kishan Dholakia

Physics

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.

Original language	English
Pages (from-to)	42-46
Number of pages	5
Journal	Photonics Research
Volume	1
Issue number	1
DOIs	https://doi.org/10.1364/PRJ.1.000042
Publication status	Published - 1 Jan 2013

Keywords

Diffractive optics
Laser beam shaping

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics

Access to Document

10.1364/PRJ.1.000042

Cite this

@article{08529b0b4bbe403ea1918c9147355079,

title = "Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]",

abstract = "We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35\% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.",

keywords = "Diffractive optics, Laser beam shaping",

author = "Xanthi Tsampoula and Michael Mazilu and Tom Vettenburg and Frank Gunn-Moore and Kishan Dholakia",

year = "2013",

month = jan,

day = "1",

doi = "10.1364/PRJ.1.000042",

language = "English",

volume = "1",

pages = "42--46",

journal = "Photonics Research",

issn = "2327-9125",

publisher = "Optica Publishing Group (formerly OSA)",

number = "1",

}

TY - JOUR

T1 - Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]

AU - Tsampoula, Xanthi

AU - Mazilu, Michael

AU - Vettenburg, Tom

AU - Gunn-Moore, Frank

AU - Dholakia, Kishan

PY - 2013/1/1

Y1 - 2013/1/1

N2 - We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.

AB - We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.

KW - Diffractive optics

KW - Laser beam shaping

UR - https://www.scopus.com/pages/publications/84891359403

U2 - 10.1364/PRJ.1.000042

DO - 10.1364/PRJ.1.000042

M3 - Article

AN - SCOPUS:84891359403

SN - 2327-9125

VL - 1

SP - 42

EP - 46

JO - Photonics Research

JF - Photonics Research

IS - 1

ER -

Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this