TY - JOUR
T1 - Enhanced liver cancer cellular response to a drug on a 3D nanostructured matrix of decellularized eggplant leaves
AU - Ahmadian, Mariye
AU - Hosseini, Saadi
AU - Alipour, Atefeh
AU - Kazemi, Javad
AU - Farrokhi, Naser
AU - Jahanfar, Mehdi
AU - Homaeigohar, Shahin
AU - Shahsavarani, Hosein
N1 - Copyright:
© 2024 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
PY - 2024/6
Y1 - 2024/6
N2 - Mimicking tissue-like microenvironments in three-dimensional (3D) cell culture systems is an appealing objective towards bridging the knowledge gap between in vitro research and in vivo applications. Through this strategy, researchers can study cell-cell interactions, cell signaling pathways, and responses to external stimuli in a more physiologically relevant setting. Accordingly, the necessary groundwork for disease modelling, drug target identification, and efficacy assessment of therapeutic compounds is realized. Moreover, almost all the ethical issues regarding the implementation of animal or human models are addressed. In this regard, here, a new cellulose-based 3D scaffold with surface trichomes and biomimetic topographical characteristics was developed via chemical decellularization of eggplant leaf (DEL). This 3D scaffold was aimed to perform as a biomimetic microenvironment of hepatocarcinoma tissue. The 3D model was assessed by seeding HepG2 cells on DEL to check the effect of prilocaine (an anticancer drug) on cancer cells. According to the diverse biological characterizations carried out in this study, cell colonies formed on DEL, and a larger efficacy of the drug was observed on the natural 3D scaffold. In conclusion, DEL as a 3D hepatocellular carcinoma (HCC) model was shown successful in chemotherapeutic drug screening.
AB - Mimicking tissue-like microenvironments in three-dimensional (3D) cell culture systems is an appealing objective towards bridging the knowledge gap between in vitro research and in vivo applications. Through this strategy, researchers can study cell-cell interactions, cell signaling pathways, and responses to external stimuli in a more physiologically relevant setting. Accordingly, the necessary groundwork for disease modelling, drug target identification, and efficacy assessment of therapeutic compounds is realized. Moreover, almost all the ethical issues regarding the implementation of animal or human models are addressed. In this regard, here, a new cellulose-based 3D scaffold with surface trichomes and biomimetic topographical characteristics was developed via chemical decellularization of eggplant leaf (DEL). This 3D scaffold was aimed to perform as a biomimetic microenvironment of hepatocarcinoma tissue. The 3D model was assessed by seeding HepG2 cells on DEL to check the effect of prilocaine (an anticancer drug) on cancer cells. According to the diverse biological characterizations carried out in this study, cell colonies formed on DEL, and a larger efficacy of the drug was observed on the natural 3D scaffold. In conclusion, DEL as a 3D hepatocellular carcinoma (HCC) model was shown successful in chemotherapeutic drug screening.
KW - Drug screening
KW - Liver cancer cell modeling
KW - Natural 3D scaffold
KW - Trichome
KW - Tumor microenvironment
UR - http://www.scopus.com/inward/record.url?scp=85193839246&partnerID=8YFLogxK
U2 - 10.1016/j.mtcomm.2024.109318
DO - 10.1016/j.mtcomm.2024.109318
M3 - Article
SN - 2352-4928
VL - 39
JO - Materials Today Communications
JF - Materials Today Communications
M1 - 109318
ER -