Enhanced liver cancer cellular response to a drug on a 3D nanostructured matrix of decellularized eggplant leaves

TY - JOUR

T1 - Enhanced liver cancer cellular response to a drug on a 3D nanostructured matrix of decellularized eggplant leaves

AU - Ahmadian, Mariye

AU - Hosseini, Saadi

AU - Alipour, Atefeh

AU - Kazemi, Javad

AU - Farrokhi, Naser

AU - Jahanfar, Mehdi

AU - Homaeigohar, Shahin

AU - Shahsavarani, Hosein

N1 - Copyright: © 2024 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.

PY - 2024/6

Y1 - 2024/6

N2 - Mimicking tissue-like microenvironments in three-dimensional (3D) cell culture systems is an appealing objective towards bridging the knowledge gap between in vitro research and in vivo applications. Through this strategy, researchers can study cell-cell interactions, cell signaling pathways, and responses to external stimuli in a more physiologically relevant setting. Accordingly, the necessary groundwork for disease modelling, drug target identification, and efficacy assessment of therapeutic compounds is realized. Moreover, almost all the ethical issues regarding the implementation of animal or human models are addressed. In this regard, here, a new cellulose-based 3D scaffold with surface trichomes and biomimetic topographical characteristics was developed via chemical decellularization of eggplant leaf (DEL). This 3D scaffold was aimed to perform as a biomimetic microenvironment of hepatocarcinoma tissue. The 3D model was assessed by seeding HepG2 cells on DEL to check the effect of prilocaine (an anticancer drug) on cancer cells. According to the diverse biological characterizations carried out in this study, cell colonies formed on DEL, and a larger efficacy of the drug was observed on the natural 3D scaffold. In conclusion, DEL as a 3D hepatocellular carcinoma (HCC) model was shown successful in chemotherapeutic drug screening.

AB - Mimicking tissue-like microenvironments in three-dimensional (3D) cell culture systems is an appealing objective towards bridging the knowledge gap between in vitro research and in vivo applications. Through this strategy, researchers can study cell-cell interactions, cell signaling pathways, and responses to external stimuli in a more physiologically relevant setting. Accordingly, the necessary groundwork for disease modelling, drug target identification, and efficacy assessment of therapeutic compounds is realized. Moreover, almost all the ethical issues regarding the implementation of animal or human models are addressed. In this regard, here, a new cellulose-based 3D scaffold with surface trichomes and biomimetic topographical characteristics was developed via chemical decellularization of eggplant leaf (DEL). This 3D scaffold was aimed to perform as a biomimetic microenvironment of hepatocarcinoma tissue. The 3D model was assessed by seeding HepG2 cells on DEL to check the effect of prilocaine (an anticancer drug) on cancer cells. According to the diverse biological characterizations carried out in this study, cell colonies formed on DEL, and a larger efficacy of the drug was observed on the natural 3D scaffold. In conclusion, DEL as a 3D hepatocellular carcinoma (HCC) model was shown successful in chemotherapeutic drug screening.

KW - Drug screening

KW - Liver cancer cell modeling

KW - Natural 3D scaffold

KW - Trichome

KW - Tumor microenvironment

UR - https://www.scopus.com/pages/publications/85193839246

U2 - 10.1016/j.mtcomm.2024.109318

DO - 10.1016/j.mtcomm.2024.109318

M3 - Article

SN - 2352-4928

VL - 39

JO - Materials Today Communications

JF - Materials Today Communications

M1 - 109318

ER -