Enhancement of image quality and imaging depth with airy light-sheet microscopy in cleared and non-cleared neural tissue

Jonathan Nylk (Lead / Corresponding author), Kaley McCluskey, Sanya Aggarwal, Javier A. Tello, Kishan Dholakia

Research output: Contribution to journalArticle

14 Citations (Scopus)
5 Downloads (Pure)

Abstract

We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.

Original languageEnglish
Pages (from-to)4021-4033
Number of pages13
JournalBiomedical Optics Express
Volume7
Issue number10
Early online date14 Sep 2016
DOIs
Publication statusPublished - 1 Oct 2016

Keywords

  • Fluorescence microscopy
  • Fourier optics and signal processing
  • Image analysis
  • Image enhancement
  • Imaging through turbid media
  • Microscopy

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