Enhancement of image quality and imaging depth with airy light-sheet microscopy in cleared and non-cleared neural tissue

TY - JOUR

T1 - Enhancement of image quality and imaging depth with airy light-sheet microscopy in cleared and non-cleared neural tissue

AU - Nylk, Jonathan

AU - McCluskey, Kaley

AU - Aggarwal, Sanya

AU - Tello, Javier A.

AU - Dholakia, Kishan

PY - 2016/10/1

Y1 - 2016/10/1

N2 - We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.

AB - We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.

KW - Fluorescence microscopy

KW - Fourier optics and signal processing

KW - Image analysis

KW - Image enhancement

KW - Imaging through turbid media

KW - Microscopy

UR - https://www.scopus.com/pages/publications/84990059436

U2 - 10.1364/BOE.7.004021

DO - 10.1364/BOE.7.004021

M3 - Article

AN - SCOPUS:84990059436

SN - 2156-7085

VL - 7

SP - 4021

EP - 4033

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 10

ER -