Environmental and genetic factors associated with Solanesol accumulation in potato leaves

Raymond Campbell, Sabine Freitag, Glenn J. Bryan, Derek Stewart, Mark A. Taylor (Lead / Corresponding author)

Research output: Contribution to journalArticle

9 Citations (Scopus)
75 Downloads (Pure)

Abstract

Solanesol is a high value 45-carbon, unsaturated, all-trans-nonaprenol isoprenoid. Recently solanesol has received particular attention because of its utility, both in its own right and as a precursor in the production of numerous compounds used in the treatment of disease states. Solanesol is found mainly in solanaceous crops such as potato, tomato, tobacco and pepper where it accumulates in the foliage. There is considerable potential to explore the extraction of solanesol from these sources as a valuable co-product. In this study we have characterized the genetic variation in leaf solanesol content in a biparental, segregating diploid potato population. We demonstrate that potato leaf solanesol content is genetically controlled and identify several quantitative trait loci associated with leaf solanesol content. Transient over-expression of genes from the methylerythritol 4-phosphate (MEP) and mevalonic acid (MVA) pathways, either singly or in combination, resulted in enhanced accumulation of solanesol in leaves of Nicotiana benthamiana, providing insights for genetically engineering the pathway. We also demonstrate that in potato, leaf solanesol content is enhanced by up to six-fold on exposure to moderately elevated temperature and show corresponding changes in expression patterns of MEP and MVA genes. Our combined approaches offer new insights into solanesol accumulation and strategies for developing a bio-refinery approach to potato production.

Original languageEnglish
Article number1263
Number of pages13
JournalFrontiers in Plant Science
Volume7
DOIs
Publication statusPublished - 25 Aug 2016

Fingerprint

potatoes
mevalonic acid
leaves
phosphates
gene overexpression
coproducts
Nicotiana benthamiana
isoprenoids
pepper
quantitative trait loci
engineering
diploidy
tobacco
tomatoes
genetic variation
carbon
crops
temperature
genes

Keywords

  • solanesol
  • environment
  • potato
  • QTL mapping
  • isoprenoid
  • heat stress

Cite this

Campbell, Raymond ; Freitag, Sabine ; Bryan, Glenn J. ; Stewart, Derek ; Taylor, Mark A. / Environmental and genetic factors associated with Solanesol accumulation in potato leaves. In: Frontiers in Plant Science. 2016 ; Vol. 7.
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Environmental and genetic factors associated with Solanesol accumulation in potato leaves. / Campbell, Raymond; Freitag, Sabine; Bryan, Glenn J.; Stewart, Derek; Taylor, Mark A. (Lead / Corresponding author).

In: Frontiers in Plant Science, Vol. 7, 1263, 25.08.2016.

Research output: Contribution to journalArticle

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T1 - Environmental and genetic factors associated with Solanesol accumulation in potato leaves

AU - Campbell, Raymond

AU - Freitag, Sabine

AU - Bryan, Glenn J.

AU - Stewart, Derek

AU - Taylor, Mark A.

N1 - This work was supported through the European Union Framework Program 7 DISCO grant 613153 (From discovery to products: A next generation pipeline for the sustainable generation of high-value plant products).

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AB - Solanesol is a high value 45-carbon, unsaturated, all-trans-nonaprenol isoprenoid. Recently solanesol has received particular attention because of its utility, both in its own right and as a precursor in the production of numerous compounds used in the treatment of disease states. Solanesol is found mainly in solanaceous crops such as potato, tomato, tobacco and pepper where it accumulates in the foliage. There is considerable potential to explore the extraction of solanesol from these sources as a valuable co-product. In this study we have characterized the genetic variation in leaf solanesol content in a biparental, segregating diploid potato population. We demonstrate that potato leaf solanesol content is genetically controlled and identify several quantitative trait loci associated with leaf solanesol content. Transient over-expression of genes from the methylerythritol 4-phosphate (MEP) and mevalonic acid (MVA) pathways, either singly or in combination, resulted in enhanced accumulation of solanesol in leaves of Nicotiana benthamiana, providing insights for genetically engineering the pathway. We also demonstrate that in potato, leaf solanesol content is enhanced by up to six-fold on exposure to moderately elevated temperature and show corresponding changes in expression patterns of MEP and MVA genes. Our combined approaches offer new insights into solanesol accumulation and strategies for developing a bio-refinery approach to potato production.

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