Enzymatic Phosphorylation of Ser in a Type I Collagen Peptide

Yimin Qiu, Erik Poppleton, Arya Mekkat, Hongtao Yu, Sourav Banerjee, Sandra E. Wiley, Jack E. Dixon, David L. Kaplan, Yu Shan Lin, Barbara Brodsky

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Phosphoproteomics studies have reported phosphorylation at multiple sites within collagen, raising the possibility that these post-translational modifications regulate the physical or biological properties of collagen. In this study, molecular dynamics simulations and experimental studies were carried out on model peptides to establish foundational principles of phosphorylation of Ser residues in collagen. A (Gly-Xaa-Yaa)11 peptide was designed to include a Ser-containing sequence from type I collagen that was reported to be phosphorylated. The physiological kinase involved in collagen phosphorylation is not known. In vitro studies showed that a model kinase ERK1 (extracellular signal-regulated protein kinase 1) would phosphorylate Ser within the consensus sequence if the collagen-like peptide is in the denatured state but not in the triple-helical state. The peptide was not a substrate for FAM20C, a kinase present in the secretory pathway, which has been shown to phosphorylate many extracellular matrix proteins. The unfolded single chain (Gly-Xaa-Yaa)11 peptide containing phosphoSer was able to refold to form a stable triple helix but at a reduced folding rate and with a small decrease in thermal stability relative to the nonphosphorylated peptide at neutral pH. These biophysical studies on model peptides provide a basis for investigations into the physiological consequences of collagen phosphorylation and the application of phosphorylation to regulate the properties of collagen biomaterials.

Original languageEnglish
Pages (from-to)2327-2335
Number of pages9
JournalBiophysical Journal
Volume115
Issue number12
DOIs
Publication statusPublished - 18 Dec 2018

Fingerprint Dive into the research topics of 'Enzymatic Phosphorylation of Ser in a Type I Collagen Peptide'. Together they form a unique fingerprint.

  • Cite this

    Qiu, Y., Poppleton, E., Mekkat, A., Yu, H., Banerjee, S., Wiley, S. E., Dixon, J. E., Kaplan, D. L., Lin, Y. S., & Brodsky, B. (2018). Enzymatic Phosphorylation of Ser in a Type I Collagen Peptide. Biophysical Journal, 115(12), 2327-2335. https://doi.org/10.1016/j.bpj.2018.11.012