Epidermal desquamation in Thiel-embalmed cadavers: histologic study

oral presentation

Research output: Contribution to conferenceOther

Abstract

Direct comparison of epidermal and dermal fingerprints may be vital to the identification of bodies that have been in water for long periods of time and their epidermal skin layer is no longer available. Epidermal desquamation occurs during the fixation of body in Thiel embalming fluid. The desquamation provides an opportunity to study the relationship between the epidermal and dermal fingerprints of Thiel-embalmed bodies. Current literature is vague on the histology of the desquamated skin layer in Thiel-embalmed bodies, therefore exposure of the dermal layer as a result of desquamation needs to be confirmed at the histological level. The desquamation that occurs as a result of the Thiel embalming process allows investigation to establish both where the separation of the skin layers occurs, but also at which point during the exposure of the skin to a wet environment this separation might be expected to happen. The aim of the study is to establish a timeframe of epidermal desquamation and describe the histology of the separation process that occurs during the Thiel embalming process. Skin of the left hand thumb (N = 8) was sampled using a standard skin biopsy punch prior to embalming and then in weekly intervals for up to six weeks after immersion in the embalming fluid. The skin was then fixed in formalin, dehydrated in ascending concentrations of ethanol, and paraffin-embedded. Microtome skin sections were stained with hematoxylin and eosin and visualised using light microscopy. The initial results confirmed complete desquamation of all epidermal layers. By week four of immersion in the embalming fluid, desquamation was completed in six out of eight individuals. As epidermal desquamation during embalming process exposes the dermal skin layer, Thiel-embalmed cadavers are suggested as suitable models for further research of dermal-epidermal fingerprint comparison.
Original languageEnglish
Publication statusPublished - 11 Sep 2018
Event7th Doctoral School of the Ecole des Sciences Criminelles - Hotel Cristal, Saignelegier, Switzerland
Duration: 10 Sep 201813 Sep 2018

Conference

Conference7th Doctoral School of the Ecole des Sciences Criminelles
CountrySwitzerland
CitySaignelegier
Period10/09/1813/09/18

Fingerprint

Cadaver
Embalming
Skin
Dermatoglyphics
Immersion
Histology
Thumb
Hematoxylin
Eosine Yellowish-(YS)
Paraffin
Formaldehyde
Microscopy
Ethanol
Hand

Keywords

  • dermal fingerprints
  • epidermal desquamation
  • Thiel embalming
  • skin histology

Cite this

Dzetkulicová, V., Langstaff, H., & Hackman, S. (2018). Epidermal desquamation in Thiel-embalmed cadavers: histologic study: oral presentation. 7th Doctoral School of the Ecole des Sciences Criminelles, Saignelegier, Switzerland.
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title = "Epidermal desquamation in Thiel-embalmed cadavers: histologic study: oral presentation",
abstract = "Direct comparison of epidermal and dermal fingerprints may be vital to the identification of bodies that have been in water for long periods of time and their epidermal skin layer is no longer available. Epidermal desquamation occurs during the fixation of body in Thiel embalming fluid. The desquamation provides an opportunity to study the relationship between the epidermal and dermal fingerprints of Thiel-embalmed bodies. Current literature is vague on the histology of the desquamated skin layer in Thiel-embalmed bodies, therefore exposure of the dermal layer as a result of desquamation needs to be confirmed at the histological level. The desquamation that occurs as a result of the Thiel embalming process allows investigation to establish both where the separation of the skin layers occurs, but also at which point during the exposure of the skin to a wet environment this separation might be expected to happen. The aim of the study is to establish a timeframe of epidermal desquamation and describe the histology of the separation process that occurs during the Thiel embalming process. Skin of the left hand thumb (N = 8) was sampled using a standard skin biopsy punch prior to embalming and then in weekly intervals for up to six weeks after immersion in the embalming fluid. The skin was then fixed in formalin, dehydrated in ascending concentrations of ethanol, and paraffin-embedded. Microtome skin sections were stained with hematoxylin and eosin and visualised using light microscopy. The initial results confirmed complete desquamation of all epidermal layers. By week four of immersion in the embalming fluid, desquamation was completed in six out of eight individuals. As epidermal desquamation during embalming process exposes the dermal skin layer, Thiel-embalmed cadavers are suggested as suitable models for further research of dermal-epidermal fingerprint comparison.",
keywords = "dermal fingerprints, epidermal desquamation, Thiel embalming, skin histology",
author = "Veronika Dzetkulicov{\'a} and Helen Langstaff and Sarah Hackman",
year = "2018",
month = "9",
day = "11",
language = "English",
note = "7th Doctoral School of the Ecole des Sciences Criminelles ; Conference date: 10-09-2018 Through 13-09-2018",

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Dzetkulicová, V, Langstaff, H & Hackman, S 2018, 'Epidermal desquamation in Thiel-embalmed cadavers: histologic study: oral presentation' 7th Doctoral School of the Ecole des Sciences Criminelles, Saignelegier, Switzerland, 10/09/18 - 13/09/18, .

Epidermal desquamation in Thiel-embalmed cadavers: histologic study : oral presentation. / Dzetkulicová, Veronika; Langstaff, Helen (Supervisor); Hackman, Sarah (Supervisor).

2018. 7th Doctoral School of the Ecole des Sciences Criminelles, Saignelegier, Switzerland.

Research output: Contribution to conferenceOther

TY - CONF

T1 - Epidermal desquamation in Thiel-embalmed cadavers: histologic study

T2 - oral presentation

AU - Dzetkulicová, Veronika

A2 - Langstaff, Helen

A2 - Hackman, Sarah

PY - 2018/9/11

Y1 - 2018/9/11

N2 - Direct comparison of epidermal and dermal fingerprints may be vital to the identification of bodies that have been in water for long periods of time and their epidermal skin layer is no longer available. Epidermal desquamation occurs during the fixation of body in Thiel embalming fluid. The desquamation provides an opportunity to study the relationship between the epidermal and dermal fingerprints of Thiel-embalmed bodies. Current literature is vague on the histology of the desquamated skin layer in Thiel-embalmed bodies, therefore exposure of the dermal layer as a result of desquamation needs to be confirmed at the histological level. The desquamation that occurs as a result of the Thiel embalming process allows investigation to establish both where the separation of the skin layers occurs, but also at which point during the exposure of the skin to a wet environment this separation might be expected to happen. The aim of the study is to establish a timeframe of epidermal desquamation and describe the histology of the separation process that occurs during the Thiel embalming process. Skin of the left hand thumb (N = 8) was sampled using a standard skin biopsy punch prior to embalming and then in weekly intervals for up to six weeks after immersion in the embalming fluid. The skin was then fixed in formalin, dehydrated in ascending concentrations of ethanol, and paraffin-embedded. Microtome skin sections were stained with hematoxylin and eosin and visualised using light microscopy. The initial results confirmed complete desquamation of all epidermal layers. By week four of immersion in the embalming fluid, desquamation was completed in six out of eight individuals. As epidermal desquamation during embalming process exposes the dermal skin layer, Thiel-embalmed cadavers are suggested as suitable models for further research of dermal-epidermal fingerprint comparison.

AB - Direct comparison of epidermal and dermal fingerprints may be vital to the identification of bodies that have been in water for long periods of time and their epidermal skin layer is no longer available. Epidermal desquamation occurs during the fixation of body in Thiel embalming fluid. The desquamation provides an opportunity to study the relationship between the epidermal and dermal fingerprints of Thiel-embalmed bodies. Current literature is vague on the histology of the desquamated skin layer in Thiel-embalmed bodies, therefore exposure of the dermal layer as a result of desquamation needs to be confirmed at the histological level. The desquamation that occurs as a result of the Thiel embalming process allows investigation to establish both where the separation of the skin layers occurs, but also at which point during the exposure of the skin to a wet environment this separation might be expected to happen. The aim of the study is to establish a timeframe of epidermal desquamation and describe the histology of the separation process that occurs during the Thiel embalming process. Skin of the left hand thumb (N = 8) was sampled using a standard skin biopsy punch prior to embalming and then in weekly intervals for up to six weeks after immersion in the embalming fluid. The skin was then fixed in formalin, dehydrated in ascending concentrations of ethanol, and paraffin-embedded. Microtome skin sections were stained with hematoxylin and eosin and visualised using light microscopy. The initial results confirmed complete desquamation of all epidermal layers. By week four of immersion in the embalming fluid, desquamation was completed in six out of eight individuals. As epidermal desquamation during embalming process exposes the dermal skin layer, Thiel-embalmed cadavers are suggested as suitable models for further research of dermal-epidermal fingerprint comparison.

KW - dermal fingerprints

KW - epidermal desquamation

KW - Thiel embalming

KW - skin histology

M3 - Other

ER -

Dzetkulicová V, Langstaff H, Hackman S. Epidermal desquamation in Thiel-embalmed cadavers: histologic study: oral presentation. 2018. 7th Doctoral School of the Ecole des Sciences Criminelles, Saignelegier, Switzerland.