EPR distance measurements in deuterated proteins

Richard Ward, A. Bowman, Erman Sozudogru, Hassane El-Mkami, Tom Owen-Hughes, David G. Norman (Lead / Corresponding author)

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    95 Citations (Scopus)

    Abstract

    One of the major problems facing distance determination by pulsed EPR, on spin-labeled proteins, has been the short relaxation time T-m. Solvent deuteration has previously been used to slow relaxation and so extend the range of distance measurement and sensitivity. We demonstrate here that deuteration of the underlying protein, as well as the solvent, extends the T-m to a considerable degree. Longer T-m gives greatly enhanced sensitivity, much extended distance measurement, more reliable distance distribution calculation and better baseline correction. (C) 2010 Elsevier Inc. All rights reserved.

    Original languageEnglish
    Pages (from-to)164-167
    Number of pages4
    JournalJournal of Magnetic Resonance Imaging
    Volume207
    Issue number1
    DOIs
    Publication statusPublished - Nov 2010

    Keywords

    • PELDOR
    • DEER
    • Relaxation
    • EPR
    • T-m
    • Deuteration
    • ELECTRON-SPIN ECHO
    • RESONANCE
    • SPECTROSCOPY

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