ERK5 pathway regulates the phosphorylation of tumour suppressor hDlg during mitosis

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    Abstract

    Human disc-large (hDlg) is a scaffold protein critical for the maintenance of cell polarity and adhesion. hDlg is thought to be a tumour suppressor that regulates the cell cycle and proliferation. However, the mechanism and pathways involved in hDlg regulation during these processes is still unclear. Here we report that hDlg is phosphorylated during mitosis, and we establish the identity of at least three residues phosphorylated in hDlg; some are previously unreported. Phosphorylation affects hDlg localisation excluding it from the contact point between the two daughter cells. Our results reveal a previously unreported pathway for hDlg phosphorylation in mitosis and show that ERK5 pathway mediates hDlg cell cycle dependent phosphorylation. This is likely to have important implications in the correct timely mitotic entry and mitosis progression. (C) 2010 Elsevier Inc. All rights reserved.

    Original languageEnglish
    Pages (from-to)84-90
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Volume399
    Issue number1
    DOIs
    Publication statusPublished - 13 Aug 2010

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